Developmental differences in endothelium-dependent responses in isolated ovine pulmonary arteries and veins

Robin H Steinhorn, F. C. Morin, S. F. Gugino, E. C. Giese, J. A. Russell

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Despite evidence for an important role for endothelium-derived relaxing factor (EDRF) in transitional circulation, previous in vitro studies of newborn pulmonary arteries have demonstrated diminished EDRF activity when compared with arteries from older animals. We studied pulmonary arteries and veins isolated from early newborn and juvenile sheep using standard tissue bath techniques. Incubation of vessels with the nitric oxide synthase inhibitor N(ω)-nitro-L-arginine (L-NNA) constricted veins but not arteries from both age groups. Further studies using preconstricted vessels revealed that arteries relaxed to acetylcholine (ACh), with significantly greater responses observed in juvenile arteries. Veins from both age groups contracted to ACh. Pretreatment with prostaglandin inhibitors (indomethacin and SQ 29,548) diminished ACh relaxations in pulmonary arteries from both age groups, greatly enhanced relaxations to ACh in newborn pulmonary veins, and depressed contractions in juvenile pulmonary veins. Removal of endothelium mechanically or functionally with prostaglandin inhibitors and L-NNA eliminated relaxations to ACh in pulmonary arteries from both age groups and resulted in contractions in veins. We conclude that isolated pulmonary veins from newborn sheep exhibit both baseline and stimulated release of EDRF, and we speculate that these venous responses may be important in the transitional pulmonary circulation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume264
Issue number6 33-6
StatePublished - 1993
Externally publishedYes

Fingerprint

Pulmonary Veins
Pulmonary Artery
Acetylcholine
Endothelium
Sheep
Endothelium-Dependent Relaxing Factors
Arteries
Age Groups
Prostaglandin Antagonists
Veins
Newborn Infant
Pulmonary Circulation
Baths
Nitric Oxide Synthase
Indomethacin
Arginine

Keywords

  • birth
  • endothelium-dependent relaxing factor
  • nitric oxide
  • prostaglandins
  • transitional circulation
  • vasodilation

ASJC Scopus subject areas

  • Physiology

Cite this

Developmental differences in endothelium-dependent responses in isolated ovine pulmonary arteries and veins. / Steinhorn, Robin H; Morin, F. C.; Gugino, S. F.; Giese, E. C.; Russell, J. A.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 264, No. 6 33-6, 1993.

Research output: Contribution to journalArticle

Steinhorn, Robin H ; Morin, F. C. ; Gugino, S. F. ; Giese, E. C. ; Russell, J. A. / Developmental differences in endothelium-dependent responses in isolated ovine pulmonary arteries and veins. In: American Journal of Physiology - Heart and Circulatory Physiology. 1993 ; Vol. 264, No. 6 33-6.
@article{6f12f1d1cff2488c909fe8f837b97094,
title = "Developmental differences in endothelium-dependent responses in isolated ovine pulmonary arteries and veins",
abstract = "Despite evidence for an important role for endothelium-derived relaxing factor (EDRF) in transitional circulation, previous in vitro studies of newborn pulmonary arteries have demonstrated diminished EDRF activity when compared with arteries from older animals. We studied pulmonary arteries and veins isolated from early newborn and juvenile sheep using standard tissue bath techniques. Incubation of vessels with the nitric oxide synthase inhibitor N(ω)-nitro-L-arginine (L-NNA) constricted veins but not arteries from both age groups. Further studies using preconstricted vessels revealed that arteries relaxed to acetylcholine (ACh), with significantly greater responses observed in juvenile arteries. Veins from both age groups contracted to ACh. Pretreatment with prostaglandin inhibitors (indomethacin and SQ 29,548) diminished ACh relaxations in pulmonary arteries from both age groups, greatly enhanced relaxations to ACh in newborn pulmonary veins, and depressed contractions in juvenile pulmonary veins. Removal of endothelium mechanically or functionally with prostaglandin inhibitors and L-NNA eliminated relaxations to ACh in pulmonary arteries from both age groups and resulted in contractions in veins. We conclude that isolated pulmonary veins from newborn sheep exhibit both baseline and stimulated release of EDRF, and we speculate that these venous responses may be important in the transitional pulmonary circulation.",
keywords = "birth, endothelium-dependent relaxing factor, nitric oxide, prostaglandins, transitional circulation, vasodilation",
author = "Steinhorn, {Robin H} and Morin, {F. C.} and Gugino, {S. F.} and Giese, {E. C.} and Russell, {J. A.}",
year = "1993",
language = "English (US)",
volume = "264",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6 33-6",

}

TY - JOUR

T1 - Developmental differences in endothelium-dependent responses in isolated ovine pulmonary arteries and veins

AU - Steinhorn, Robin H

AU - Morin, F. C.

AU - Gugino, S. F.

AU - Giese, E. C.

AU - Russell, J. A.

PY - 1993

Y1 - 1993

N2 - Despite evidence for an important role for endothelium-derived relaxing factor (EDRF) in transitional circulation, previous in vitro studies of newborn pulmonary arteries have demonstrated diminished EDRF activity when compared with arteries from older animals. We studied pulmonary arteries and veins isolated from early newborn and juvenile sheep using standard tissue bath techniques. Incubation of vessels with the nitric oxide synthase inhibitor N(ω)-nitro-L-arginine (L-NNA) constricted veins but not arteries from both age groups. Further studies using preconstricted vessels revealed that arteries relaxed to acetylcholine (ACh), with significantly greater responses observed in juvenile arteries. Veins from both age groups contracted to ACh. Pretreatment with prostaglandin inhibitors (indomethacin and SQ 29,548) diminished ACh relaxations in pulmonary arteries from both age groups, greatly enhanced relaxations to ACh in newborn pulmonary veins, and depressed contractions in juvenile pulmonary veins. Removal of endothelium mechanically or functionally with prostaglandin inhibitors and L-NNA eliminated relaxations to ACh in pulmonary arteries from both age groups and resulted in contractions in veins. We conclude that isolated pulmonary veins from newborn sheep exhibit both baseline and stimulated release of EDRF, and we speculate that these venous responses may be important in the transitional pulmonary circulation.

AB - Despite evidence for an important role for endothelium-derived relaxing factor (EDRF) in transitional circulation, previous in vitro studies of newborn pulmonary arteries have demonstrated diminished EDRF activity when compared with arteries from older animals. We studied pulmonary arteries and veins isolated from early newborn and juvenile sheep using standard tissue bath techniques. Incubation of vessels with the nitric oxide synthase inhibitor N(ω)-nitro-L-arginine (L-NNA) constricted veins but not arteries from both age groups. Further studies using preconstricted vessels revealed that arteries relaxed to acetylcholine (ACh), with significantly greater responses observed in juvenile arteries. Veins from both age groups contracted to ACh. Pretreatment with prostaglandin inhibitors (indomethacin and SQ 29,548) diminished ACh relaxations in pulmonary arteries from both age groups, greatly enhanced relaxations to ACh in newborn pulmonary veins, and depressed contractions in juvenile pulmonary veins. Removal of endothelium mechanically or functionally with prostaglandin inhibitors and L-NNA eliminated relaxations to ACh in pulmonary arteries from both age groups and resulted in contractions in veins. We conclude that isolated pulmonary veins from newborn sheep exhibit both baseline and stimulated release of EDRF, and we speculate that these venous responses may be important in the transitional pulmonary circulation.

KW - birth

KW - endothelium-dependent relaxing factor

KW - nitric oxide

KW - prostaglandins

KW - transitional circulation

KW - vasodilation

UR - http://www.scopus.com/inward/record.url?scp=0027321021&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027321021&partnerID=8YFLogxK

M3 - Article

C2 - 8322947

AN - SCOPUS:0027321021

VL - 264

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 6 33-6

ER -