Developmental changes in proximal tubule tight junction proteins

Maha Haddad, Fangming Lin, Vangipuram Dwarakanath, Kimberly Cordes, Michel Baum

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We demonstrated previously that neonatal proximal tubules have a lower passive paracellular permeability to chloride ions and higher resistance than that of adult proximal tubules. In addition, administration of thyroid hormone to neonates, before the normal maturational increase in serum thyroid hormone levels, prematurely accelerates the developmental increase in chloride permeability to adult levels. To test the hypothesis that there is a maturational change in tight junction proteins and that thyroid hormone mediates these changes, we examined the two known tight junction proteins present in proximal tubules, occludin and claudin 2. Using immunoblot and immunohistochemistry, we demonstrated that claudin 2 has a 4-fold greater abundance in neonatal proximal tubules than in adult tubules. Occludin, however, has a 4-fold greater expression in adult tubules than in neonatal tubules. Administration of thyroid hormone to neonates did not affect claudin 2 expression, occludin expression, or the transepithelial resistance in rat proximal tubule cells in vitro. In conclusion, there are postnatal maturational changes in tight junction proteins. The factors that cause these maturational changes are unknown but unlikely to be due solely to the maturational increase in thyroid hormone.

Original languageEnglish (US)
Pages (from-to)453-457
Number of pages5
JournalPediatric Research
Volume57
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Tight Junction Proteins
Thyroid Hormones
Claudin-2
Occludin
Chlorides
Permeability
Immunohistochemistry
Ions
Serum

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Developmental changes in proximal tubule tight junction proteins. / Haddad, Maha; Lin, Fangming; Dwarakanath, Vangipuram; Cordes, Kimberly; Baum, Michel.

In: Pediatric Research, Vol. 57, No. 3, 03.2005, p. 453-457.

Research output: Contribution to journalArticle

Haddad, Maha ; Lin, Fangming ; Dwarakanath, Vangipuram ; Cordes, Kimberly ; Baum, Michel. / Developmental changes in proximal tubule tight junction proteins. In: Pediatric Research. 2005 ; Vol. 57, No. 3. pp. 453-457.
@article{704af78c89d04e458df10ece3d91f0a1,
title = "Developmental changes in proximal tubule tight junction proteins",
abstract = "We demonstrated previously that neonatal proximal tubules have a lower passive paracellular permeability to chloride ions and higher resistance than that of adult proximal tubules. In addition, administration of thyroid hormone to neonates, before the normal maturational increase in serum thyroid hormone levels, prematurely accelerates the developmental increase in chloride permeability to adult levels. To test the hypothesis that there is a maturational change in tight junction proteins and that thyroid hormone mediates these changes, we examined the two known tight junction proteins present in proximal tubules, occludin and claudin 2. Using immunoblot and immunohistochemistry, we demonstrated that claudin 2 has a 4-fold greater abundance in neonatal proximal tubules than in adult tubules. Occludin, however, has a 4-fold greater expression in adult tubules than in neonatal tubules. Administration of thyroid hormone to neonates did not affect claudin 2 expression, occludin expression, or the transepithelial resistance in rat proximal tubule cells in vitro. In conclusion, there are postnatal maturational changes in tight junction proteins. The factors that cause these maturational changes are unknown but unlikely to be due solely to the maturational increase in thyroid hormone.",
author = "Maha Haddad and Fangming Lin and Vangipuram Dwarakanath and Kimberly Cordes and Michel Baum",
year = "2005",
month = "3",
doi = "10.1203/01.PDR.0000151354.07752.9B",
language = "English (US)",
volume = "57",
pages = "453--457",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Developmental changes in proximal tubule tight junction proteins

AU - Haddad, Maha

AU - Lin, Fangming

AU - Dwarakanath, Vangipuram

AU - Cordes, Kimberly

AU - Baum, Michel

PY - 2005/3

Y1 - 2005/3

N2 - We demonstrated previously that neonatal proximal tubules have a lower passive paracellular permeability to chloride ions and higher resistance than that of adult proximal tubules. In addition, administration of thyroid hormone to neonates, before the normal maturational increase in serum thyroid hormone levels, prematurely accelerates the developmental increase in chloride permeability to adult levels. To test the hypothesis that there is a maturational change in tight junction proteins and that thyroid hormone mediates these changes, we examined the two known tight junction proteins present in proximal tubules, occludin and claudin 2. Using immunoblot and immunohistochemistry, we demonstrated that claudin 2 has a 4-fold greater abundance in neonatal proximal tubules than in adult tubules. Occludin, however, has a 4-fold greater expression in adult tubules than in neonatal tubules. Administration of thyroid hormone to neonates did not affect claudin 2 expression, occludin expression, or the transepithelial resistance in rat proximal tubule cells in vitro. In conclusion, there are postnatal maturational changes in tight junction proteins. The factors that cause these maturational changes are unknown but unlikely to be due solely to the maturational increase in thyroid hormone.

AB - We demonstrated previously that neonatal proximal tubules have a lower passive paracellular permeability to chloride ions and higher resistance than that of adult proximal tubules. In addition, administration of thyroid hormone to neonates, before the normal maturational increase in serum thyroid hormone levels, prematurely accelerates the developmental increase in chloride permeability to adult levels. To test the hypothesis that there is a maturational change in tight junction proteins and that thyroid hormone mediates these changes, we examined the two known tight junction proteins present in proximal tubules, occludin and claudin 2. Using immunoblot and immunohistochemistry, we demonstrated that claudin 2 has a 4-fold greater abundance in neonatal proximal tubules than in adult tubules. Occludin, however, has a 4-fold greater expression in adult tubules than in neonatal tubules. Administration of thyroid hormone to neonates did not affect claudin 2 expression, occludin expression, or the transepithelial resistance in rat proximal tubule cells in vitro. In conclusion, there are postnatal maturational changes in tight junction proteins. The factors that cause these maturational changes are unknown but unlikely to be due solely to the maturational increase in thyroid hormone.

UR - http://www.scopus.com/inward/record.url?scp=14344251315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14344251315&partnerID=8YFLogxK

U2 - 10.1203/01.PDR.0000151354.07752.9B

DO - 10.1203/01.PDR.0000151354.07752.9B

M3 - Article

VL - 57

SP - 453

EP - 457

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 3

ER -