Development of toxicity identification evaluation procedures for pyrethroid detection using esterase activity

Craig E. Wheelock, Jeff L. Miller, Mike J. Miller, Shirley J. Gee, Guomin Shan, Bruce D. Hammock

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Recent agrochemical usage patterns suggest that the use of organophosphate (OP) pesticides will decrease, resulting in a concomitant increase in pyrethroid usage. Pyrethroids are known for their potential toxicity to aquatic invertebrates and many fish species. Current toxicity identification evaluation (TIE) techniques are able to detect OPs, but have not been optimized for pyrethroids. Organophosphate identification methods depend upon the use of piperonyl butoxide (PBO) to identify OP-induced toxicity. However, the use of PBO in TIE assays will be confounded by the co-occurrence of OPs and pyrethroids in receiving waters. It is necessary, therefore, to develop new TIE procedures for pyrethroids. This study evaluated the use of a pyrethroid-specific antibody, PBO, and carboxylesterase activity to identify pyrethroid toxicity in aquatic toxicity testing with Ceriodaphnia dubia. The antibody caused significant mortality to the C. dubia. Piperonyl butoxide synergized pyrethroid-associated toxicity, but this effect may be difficult to interpret in the presence of OPs and pyrethroids. Carboxylesterase activity removed pyrethroid-associated toxicity in a dose-dependent manner and did not compromise OP toxicity, suggesting that carboxylesterase treatment will not interfere with TIE OP detection methods. These results indicate that the addition of carboxylesterase to TIE procedures can be used to detect pyrethroids in aquatic samples.

Original languageEnglish (US)
Pages (from-to)2699-2708
Number of pages10
JournalEnvironmental Toxicology and Chemistry
Volume23
Issue number11
DOIs
StatePublished - Nov 2004

Fingerprint

Pyrethrins
pyrethroid
Esterases
Toxicity
toxicity
Piperonyl Butoxide
Organophosphates
Carboxylesterase
organophosphate
antibody
evaluation
detection
Agrochemicals
Antibodies
identification method
Invertebrates
agrochemical
Pesticides
detection method
Fish

Keywords

  • Ceriodaphnia dubia
  • Esterase
  • Organophosphate
  • Pyrethroid
  • Toxicity identification evaluation

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Development of toxicity identification evaluation procedures for pyrethroid detection using esterase activity. / Wheelock, Craig E.; Miller, Jeff L.; Miller, Mike J.; Gee, Shirley J.; Shan, Guomin; Hammock, Bruce D.

In: Environmental Toxicology and Chemistry, Vol. 23, No. 11, 11.2004, p. 2699-2708.

Research output: Contribution to journalArticle

Wheelock, Craig E. ; Miller, Jeff L. ; Miller, Mike J. ; Gee, Shirley J. ; Shan, Guomin ; Hammock, Bruce D. / Development of toxicity identification evaluation procedures for pyrethroid detection using esterase activity. In: Environmental Toxicology and Chemistry. 2004 ; Vol. 23, No. 11. pp. 2699-2708.
@article{415d19685df94b278ce5f5de64d5eb45,
title = "Development of toxicity identification evaluation procedures for pyrethroid detection using esterase activity",
abstract = "Recent agrochemical usage patterns suggest that the use of organophosphate (OP) pesticides will decrease, resulting in a concomitant increase in pyrethroid usage. Pyrethroids are known for their potential toxicity to aquatic invertebrates and many fish species. Current toxicity identification evaluation (TIE) techniques are able to detect OPs, but have not been optimized for pyrethroids. Organophosphate identification methods depend upon the use of piperonyl butoxide (PBO) to identify OP-induced toxicity. However, the use of PBO in TIE assays will be confounded by the co-occurrence of OPs and pyrethroids in receiving waters. It is necessary, therefore, to develop new TIE procedures for pyrethroids. This study evaluated the use of a pyrethroid-specific antibody, PBO, and carboxylesterase activity to identify pyrethroid toxicity in aquatic toxicity testing with Ceriodaphnia dubia. The antibody caused significant mortality to the C. dubia. Piperonyl butoxide synergized pyrethroid-associated toxicity, but this effect may be difficult to interpret in the presence of OPs and pyrethroids. Carboxylesterase activity removed pyrethroid-associated toxicity in a dose-dependent manner and did not compromise OP toxicity, suggesting that carboxylesterase treatment will not interfere with TIE OP detection methods. These results indicate that the addition of carboxylesterase to TIE procedures can be used to detect pyrethroids in aquatic samples.",
keywords = "Ceriodaphnia dubia, Esterase, Organophosphate, Pyrethroid, Toxicity identification evaluation",
author = "Wheelock, {Craig E.} and Miller, {Jeff L.} and Miller, {Mike J.} and Gee, {Shirley J.} and Guomin Shan and Hammock, {Bruce D.}",
year = "2004",
month = "11",
doi = "10.1897/03-544",
language = "English (US)",
volume = "23",
pages = "2699--2708",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "John Wiley and Sons Ltd",
number = "11",

}

TY - JOUR

T1 - Development of toxicity identification evaluation procedures for pyrethroid detection using esterase activity

AU - Wheelock, Craig E.

AU - Miller, Jeff L.

AU - Miller, Mike J.

AU - Gee, Shirley J.

AU - Shan, Guomin

AU - Hammock, Bruce D.

PY - 2004/11

Y1 - 2004/11

N2 - Recent agrochemical usage patterns suggest that the use of organophosphate (OP) pesticides will decrease, resulting in a concomitant increase in pyrethroid usage. Pyrethroids are known for their potential toxicity to aquatic invertebrates and many fish species. Current toxicity identification evaluation (TIE) techniques are able to detect OPs, but have not been optimized for pyrethroids. Organophosphate identification methods depend upon the use of piperonyl butoxide (PBO) to identify OP-induced toxicity. However, the use of PBO in TIE assays will be confounded by the co-occurrence of OPs and pyrethroids in receiving waters. It is necessary, therefore, to develop new TIE procedures for pyrethroids. This study evaluated the use of a pyrethroid-specific antibody, PBO, and carboxylesterase activity to identify pyrethroid toxicity in aquatic toxicity testing with Ceriodaphnia dubia. The antibody caused significant mortality to the C. dubia. Piperonyl butoxide synergized pyrethroid-associated toxicity, but this effect may be difficult to interpret in the presence of OPs and pyrethroids. Carboxylesterase activity removed pyrethroid-associated toxicity in a dose-dependent manner and did not compromise OP toxicity, suggesting that carboxylesterase treatment will not interfere with TIE OP detection methods. These results indicate that the addition of carboxylesterase to TIE procedures can be used to detect pyrethroids in aquatic samples.

AB - Recent agrochemical usage patterns suggest that the use of organophosphate (OP) pesticides will decrease, resulting in a concomitant increase in pyrethroid usage. Pyrethroids are known for their potential toxicity to aquatic invertebrates and many fish species. Current toxicity identification evaluation (TIE) techniques are able to detect OPs, but have not been optimized for pyrethroids. Organophosphate identification methods depend upon the use of piperonyl butoxide (PBO) to identify OP-induced toxicity. However, the use of PBO in TIE assays will be confounded by the co-occurrence of OPs and pyrethroids in receiving waters. It is necessary, therefore, to develop new TIE procedures for pyrethroids. This study evaluated the use of a pyrethroid-specific antibody, PBO, and carboxylesterase activity to identify pyrethroid toxicity in aquatic toxicity testing with Ceriodaphnia dubia. The antibody caused significant mortality to the C. dubia. Piperonyl butoxide synergized pyrethroid-associated toxicity, but this effect may be difficult to interpret in the presence of OPs and pyrethroids. Carboxylesterase activity removed pyrethroid-associated toxicity in a dose-dependent manner and did not compromise OP toxicity, suggesting that carboxylesterase treatment will not interfere with TIE OP detection methods. These results indicate that the addition of carboxylesterase to TIE procedures can be used to detect pyrethroids in aquatic samples.

KW - Ceriodaphnia dubia

KW - Esterase

KW - Organophosphate

KW - Pyrethroid

KW - Toxicity identification evaluation

UR - http://www.scopus.com/inward/record.url?scp=5444269152&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=5444269152&partnerID=8YFLogxK

U2 - 10.1897/03-544

DO - 10.1897/03-544

M3 - Article

C2 - 15559286

AN - SCOPUS:5444269152

VL - 23

SP - 2699

EP - 2708

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 11

ER -