Development of Tetramethylenedisulfotetramine (TETS) Hapten Library: Synthesis, Electrophysiological Studies, and Immune Response in Rabbits.

Bogdan Barnych, Natalia Vasylieva, Tom Joseph, Susan Hulsizer, Hai M. Nguyen, Tomas Cajka, Isaac N Pessah, Heike Wulff, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

There is a need for fast detection methods for the banned rodenticide tetramethylenedisulfotetramine (TETS), a highly potent blocker of the γ-aminobutyric acid (GABAA) receptors. General synthetic approach toward two groups of analogues was developed. Screening of the resulting library of compounds by FLIPR or whole-cell voltage-clamp revealed that, despite the structural differences, some of the TETS analogues retained GABAA receptor inhibition; however, their potency was an order of magnitude lower. Antibodies raised in rabbits against some of the TETS analogues conjugated to protein recognized free TETS and will be used for the development of an immunoassay for TETS.

Original languageEnglish (US)
Pages (from-to)8466-8472
Number of pages7
JournalChemistry - A European Journal
Volume23
Issue number35
DOIs
StatePublished - Jun 22 2017

Keywords

  • antibodies
  • cage convulsants
  • GABA
  • neurotoxicity
  • poly-heterocycles
  • tetramine

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Development of Tetramethylenedisulfotetramine (TETS) Hapten Library: Synthesis, Electrophysiological Studies, and Immune Response in Rabbits.'. Together they form a unique fingerprint.

Cite this