Development of simian immunodeficiency virus isolation, titration, and neutralization assays which use whole blood from rhesus monkeys and an antigen capture enzyme-linked immunosorbent assay

B. L. Lohman, J. Higgins, Marta Marthas, P. A. Marx, Niels C Pedersen

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Abstract

Assays that use rhesus macaque whole blood and an antigen capture enzyme-linked immunosorbent assay for the simian immunodeficiency virus (SIV) p27 core protein were developed for the isolation of SIV from the blood of infected animals, the titration of infectivity of SIV inocula, and the quantitation of virus neutralizing antibodies in serum. These assays required small amounts of whole blood, were adaptable to a microtiter format, and used substrates mainly of rhesus macaque origin.

Original languageEnglish (US)
Pages (from-to)2187-2192
Number of pages6
JournalJournal of Clinical Microbiology
Volume29
Issue number10
StatePublished - 1991

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Simian Immunodeficiency Virus
Macaca mulatta
Enzyme-Linked Immunosorbent Assay
Antigens
Viral Core Proteins
Neutralizing Antibodies
Viruses
Serum

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

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AU - Higgins, J.

AU - Marthas, Marta

AU - Marx, P. A.

AU - Pedersen, Niels C

PY - 1991

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