Abstract
Assays that use rhesus macaque whole blood and an antigen capture enzyme-linked immunosorbent assay for the simian immunodeficiency virus (SIV) p27 core protein were developed for the isolation of SIV from the blood of infected animals, the titration of infectivity of SIV inocula, and the quantitation of virus neutralizing antibodies in serum. These assays required small amounts of whole blood, were adaptable to a microtiter format, and used substrates mainly of rhesus macaque origin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2187-2192 |
| Number of pages | 6 |
| Journal | Journal of Clinical Microbiology |
| Volume | 29 |
| Issue number | 10 |
| State | Published - 1991 |
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ASJC Scopus subject areas
- Microbiology (medical)
Cite this
Development of simian immunodeficiency virus isolation, titration, and neutralization assays which use whole blood from rhesus monkeys and an antigen capture enzyme-linked immunosorbent assay. / Lohman, B. L.; Higgins, J.; Marthas, Marta; Marx, P. A.; Pedersen, Niels C.
In: Journal of Clinical Microbiology, Vol. 29, No. 10, 1991, p. 2187-2192.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of simian immunodeficiency virus isolation, titration, and neutralization assays which use whole blood from rhesus monkeys and an antigen capture enzyme-linked immunosorbent assay
AU - Lohman, B. L.
AU - Higgins, J.
AU - Marthas, Marta
AU - Marx, P. A.
AU - Pedersen, Niels C
PY - 1991
Y1 - 1991
N2 - Assays that use rhesus macaque whole blood and an antigen capture enzyme-linked immunosorbent assay for the simian immunodeficiency virus (SIV) p27 core protein were developed for the isolation of SIV from the blood of infected animals, the titration of infectivity of SIV inocula, and the quantitation of virus neutralizing antibodies in serum. These assays required small amounts of whole blood, were adaptable to a microtiter format, and used substrates mainly of rhesus macaque origin.
AB - Assays that use rhesus macaque whole blood and an antigen capture enzyme-linked immunosorbent assay for the simian immunodeficiency virus (SIV) p27 core protein were developed for the isolation of SIV from the blood of infected animals, the titration of infectivity of SIV inocula, and the quantitation of virus neutralizing antibodies in serum. These assays required small amounts of whole blood, were adaptable to a microtiter format, and used substrates mainly of rhesus macaque origin.
UR - http://www.scopus.com/inward/record.url?scp=0025991767&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025991767&partnerID=8YFLogxK
M3 - Article
C2 - 1939569
AN - SCOPUS:0025991767
VL - 29
SP - 2187
EP - 2192
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 10
ER -