Development of restriction fragment-length polymorphism method to differentiate five subtypes of feline immunodeficiency virus

Kyoko Kurosawa, Yasuhiro Ikeda, Takayuki Miyazawa, Yoshihiro Izumiya, Yorihiro Nishimura, Kazuya Nakamura, Eiji Sato, Takeshi Mikami, Chieko Kai, Eiji Takahashi

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Feline immunodeficiency virus (FIV) isolates have been classified into five subtypes (A to E) based on the sequences of the env variable V3 to V5 region. In this study, we sequenced a partial gag region of 4 and 3 isolates belonging to subtypes C and E, respectively. Phylogenetic analysis revealed that the branching pattern based on the region was similar to that based on the env V3 to V5 region. Here, we propose a protocol to differentiate five subtypes by polymerase chain reaction amplifying 329 bp within the region followed by restriction fragment-length polymorphism analysis using four restriction enzymes.

Original languageEnglish (US)
Pages (from-to)817-820
Number of pages4
JournalMicrobiology and Immunology
Volume43
Issue number8
StatePublished - 1999
Externally publishedYes

Keywords

  • Feline immunodeficiency virus
  • Polymerase chain reaction
  • Restriction fragment-length polymorphism

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

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  • Cite this

    Kurosawa, K., Ikeda, Y., Miyazawa, T., Izumiya, Y., Nishimura, Y., Nakamura, K., Sato, E., Mikami, T., Kai, C., & Takahashi, E. (1999). Development of restriction fragment-length polymorphism method to differentiate five subtypes of feline immunodeficiency virus. Microbiology and Immunology, 43(8), 817-820.