Development of resorbable nanocomposite tracheal and bronchial scaffolds for paediatric applications

G. Z. Teoh, C. Crowley, M. A. Birchall, A. M. Seifalian

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Congenital tracheal defects and prolonged intubation following premature birth have resulted in an unmet clinical need for tracheal replacement. Advances in stem cell technology, tissue engineering and material sciences have inspired the development of a resorbable, nanocomposite tracheal and bronchial scaffold. Methods A bifurcated scaffold was designed and constructed using a novel, resorbable nanocomposite polymer, polyhedral oligomeric silsesquioxane poly(Ïμ-caprolactone) urea urethane (POSS-PCL). Material characterization studies included tensile strength, suture retention and surface characteristics. Bone marrow-derived mesenchymal stem cells (bmMSCs) and human tracheobronchial epithelial cells (HBECs) were cultured on POSS-PCL for up to 14 days, and metabolic activity and cell morphology were assessed. Quantum dots conjugated to RGD (l-arginine, glycine and l-aspartic acid) tripeptides and anticollagen type I antibody were then employed to observe cell migration throughout the scaffold. Results POSS-PCL exhibited good mechanical properties, and the relationship between the solid elastomer and foam elastomer of POSS-PCL was comparable to that between the cartilaginous U-shaped rings and interconnective cartilage of the native human trachea. Good suture retention was also achieved. Cell attachment and a significant, steady increase in proliferation were observed for both cell types (bmMSCs, P=0·001; HBECs, P=0·003). Quantum dot imaging illustrated adequate cell penetration throughout the scaffold, which was confirmed by scanning electron microscopy. Conclusion This mechanically viable scaffold successfully supports bmMSC and HBEC attachment and proliferation, demonstrating its potential as a tissue-engineered solution to tracheal replacement.

Original languageEnglish (US)
JournalBritish Journal of Surgery
Volume102
Issue number2
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Nanocomposites
Elastomers
Pediatrics
Quantum Dots
Epithelial Cells
Mesenchymal Stromal Cells
Sutures
Epithelial Attachment
Tensile Strength
Urethane
Premature Birth
Tissue Engineering
Trachea
Population Groups
Intubation
Aspartic Acid
Bone Marrow Cells
Electron Scanning Microscopy
Glycine
Cartilage

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Development of resorbable nanocomposite tracheal and bronchial scaffolds for paediatric applications. / Teoh, G. Z.; Crowley, C.; Birchall, M. A.; Seifalian, A. M.

In: British Journal of Surgery, Vol. 102, No. 2, 01.01.2015.

Research output: Contribution to journalArticle

Teoh, G. Z. ; Crowley, C. ; Birchall, M. A. ; Seifalian, A. M. / Development of resorbable nanocomposite tracheal and bronchial scaffolds for paediatric applications. In: British Journal of Surgery. 2015 ; Vol. 102, No. 2.
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