Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce

I. Paran, Richard W Michelmore

Research output: Contribution to journalArticle

1042 Scopus citations

Abstract

Sequence characterized amplified regions (SCARs) were derived from eight random amplified polymorphic DNA (RAPD) markers linked to disease resistance genes in lettuce. SCARs are PCR-based markers that represent single, genetically defined loci that are identified by PCR amplification of genomic DNA with pairs of specific oligonucleotide primers; they may contain high-copy, dispersed genomic sequences within the amplified region. Amplified RAPD products were cloned and sequenced. The sequence was used to design 24-mer oligonucleotide primers for each end. All pairs of SCAR primers resulted in the amplification of single major bands the same size as the RAPD fragment cloned. Polymorphism was either retained as the presence or absence of amplification of the band or appeared as length polymorphisms that converted dominant RAPD loci into codominant SCAR markers. This study provided information on the molecular basis of RAPD markers. The amplified fragment contained no obvious repeated sequences beyond the primer sequence. Five out of eight pairs of SCAR primers amplified an alternate allele from both parents of the mapping population; therefore, the original RAPD polymorphism was likely due to mismatch at the primer sites.

Original languageEnglish (US)
Pages (from-to)985-993
Number of pages9
JournalTheoretical And Applied Genetics
Volume85
Issue number8
DOIs
StatePublished - Feb 1993

Keywords

  • Disease resistance
  • Downy mildew
  • Lettuce
  • Molecular marker

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Fingerprint Dive into the research topics of 'Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce'. Together they form a unique fingerprint.

  • Cite this