Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses.

B. D. Hammock, B. F. McCutchen, J. Beetham, Prabhakara V Choudary, E. Fowler, R. Ichinose, V. K. Ward, J. M. Vickers, B. C. Bonning, L. G. Harshman

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

As supplements to classical chemical insecticides, two approaches to develop recombinant baculovirus insecticides are described. In one approach an insect-specific toxin is expressed leading to a dramatic reduction in time to death. In the second approach an insect juvenile hormone esterase is expressed which leads to a reduction in feeding. Modifications of the wildtype esterase led to viruses which reduced the time to death as effectively as did the toxin-expressing virus. In both cases existing recombinant viruses are viewed as leads, and approaches to further improvement in the engineered viruses are suggested. Many of these approaches are based on analogy with the development of classical synthetic insecticides. Using these viruses as examples, the potential utility and limitations of recombinant viruses and other biological insecticides are discussed.

Original languageEnglish (US)
Pages (from-to)315-344
Number of pages30
JournalArchives of Insect Biochemistry and Physiology
Volume22
Issue number3-4
StatePublished - 1993

Fingerprint

viral insecticides
Nucleopolyhedrovirus
Baculoviridae
Insecticides
Viruses
Insects
toxins
viruses
insects
Enzymes
enzymes
insecticides
Insect Hormones
juvenile hormone esterase
insect hormones
death
biopesticides
Esterases
esterases

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

Cite this

Hammock, B. D., McCutchen, B. F., Beetham, J., Choudary, P. V., Fowler, E., Ichinose, R., ... Harshman, L. G. (1993). Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses. Archives of Insect Biochemistry and Physiology, 22(3-4), 315-344.

Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses. / Hammock, B. D.; McCutchen, B. F.; Beetham, J.; Choudary, Prabhakara V; Fowler, E.; Ichinose, R.; Ward, V. K.; Vickers, J. M.; Bonning, B. C.; Harshman, L. G.

In: Archives of Insect Biochemistry and Physiology, Vol. 22, No. 3-4, 1993, p. 315-344.

Research output: Contribution to journalArticle

Hammock, BD, McCutchen, BF, Beetham, J, Choudary, PV, Fowler, E, Ichinose, R, Ward, VK, Vickers, JM, Bonning, BC & Harshman, LG 1993, 'Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses.', Archives of Insect Biochemistry and Physiology, vol. 22, no. 3-4, pp. 315-344.
Hammock, B. D. ; McCutchen, B. F. ; Beetham, J. ; Choudary, Prabhakara V ; Fowler, E. ; Ichinose, R. ; Ward, V. K. ; Vickers, J. M. ; Bonning, B. C. ; Harshman, L. G. / Development of recombinant viral insecticides by expression of an insect-specific toxin and insect-specific enzyme in nuclear polyhedrosis viruses. In: Archives of Insect Biochemistry and Physiology. 1993 ; Vol. 22, No. 3-4. pp. 315-344.
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