Development of rapid mercury assays. Synthesis of sulfur- and mercury-containing conjugates

Ferenc Szurdoki, Horacio Kido, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We have devised rapid analyses for mercury exploiting the high affinity of dithiocarbamate chelators for the mercuric ion. Our first assay is based on a sandwich chelate formed by a ligand supported on the well of an ELISA plate, Hg2+ ion of the investigated sample, and another ligand bound to a reporter enzyme. The second assay utilizes competitive binding of the analyte Hg2+ ions versus an organomercury conjugate to a chelating conjugate. Low ppb sensitivity and high selectivity for Hg2+ ions have been achieved in our pilot studies.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalBioconjugate Chemistry
Volume6
Issue number2
StatePublished - 1995

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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