Development of rapid mercury assays. Synthesis of sulfur- and mercury-containing conjugates

Ferenc Szurdoki, Horacio Kido, Bruce D. Hammock

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We have devised rapid analyses for mercury exploiting the high affinity of dithiocarbamate chelators for the mercuric ion. Our first assay is based on a sandwich chelate formed by a ligand supported on the well of an ELISA plate, Hg2+ ion of the investigated sample, and another ligand bound to a reporter enzyme. The second assay utilizes competitive binding of the analyte Hg2+ ions versus an organomercury conjugate to a chelating conjugate. Low ppb sensitivity and high selectivity for Hg2+ ions have been achieved in our pilot studies.

Original languageEnglish (US)
Pages (from-to)145-149
Number of pages5
JournalBioconjugate Chemistry
Volume6
Issue number2
StatePublished - 1995

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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