Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products

Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL -1. The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.

Original languageEnglish (US)
Pages (from-to)8441-8447
Number of pages7
JournalAnalytical Chemistry
Volume86
Issue number16
DOIs
StatePublished - Aug 19 2014

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Bacteriophages
Pesticides
Amplification
Assays
Fenitrothion
Methyl Parathion
Color
Pesticide Residues
Immunosorbents
Peptides
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products. / Hua, Xiude; Yin, Wei; Shi, Haiyan; Li, Ming; Wang, Yanru; Wang, Hong; Ye, Yonghao; Kim, Hee Joo; Gee, Shirley J.; Wang, Minghua; Liu, Fengquan; Hammock, Bruce D.

In: Analytical Chemistry, Vol. 86, No. 16, 19.08.2014, p. 8441-8447.

Research output: Contribution to journalArticle

Hua, X, Yin, W, Shi, H, Li, M, Wang, Y, Wang, H, Ye, Y, Kim, HJ, Gee, SJ, Wang, M, Liu, F & Hammock, BD 2014, 'Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products', Analytical Chemistry, vol. 86, no. 16, pp. 8441-8447. https://doi.org/10.1021/ac5020657
Hua, Xiude ; Yin, Wei ; Shi, Haiyan ; Li, Ming ; Wang, Yanru ; Wang, Hong ; Ye, Yonghao ; Kim, Hee Joo ; Gee, Shirley J. ; Wang, Minghua ; Liu, Fengquan ; Hammock, Bruce D. / Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products. In: Analytical Chemistry. 2014 ; Vol. 86, No. 16. pp. 8441-8447.
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AU - Ye, Yonghao

AU - Kim, Hee Joo

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AU - Hammock, Bruce D.

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