Abstract
Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL -1. The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8441-8447 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 86 |
| Issue number | 16 |
| DOIs | |
| State | Published - Aug 19 2014 |
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- Analytical Chemistry
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Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products. / Hua, Xiude; Yin, Wei; Shi, Haiyan; Li, Ming; Wang, Yanru; Wang, Hong; Ye, Yonghao; Kim, Hee Joo; Gee, Shirley J.; Wang, Minghua; Liu, Fengquan; Hammock, Bruce D.
In: Analytical Chemistry, Vol. 86, No. 16, 19.08.2014, p. 8441-8447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products
AU - Hua, Xiude
AU - Yin, Wei
AU - Shi, Haiyan
AU - Li, Ming
AU - Wang, Yanru
AU - Wang, Hong
AU - Ye, Yonghao
AU - Kim, Hee Joo
AU - Gee, Shirley J.
AU - Wang, Minghua
AU - Liu, Fengquan
AU - Hammock, Bruce D.
PY - 2014/8/19
Y1 - 2014/8/19
N2 - Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL -1. The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.
AB - Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL -1. The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.
UR - http://www.scopus.com/inward/record.url?scp=84906228320&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84906228320&partnerID=8YFLogxK
U2 - 10.1021/ac5020657
DO - 10.1021/ac5020657
M3 - Article
C2 - 25135320
AN - SCOPUS:84906228320
VL - 86
SP - 8441
EP - 8447
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 16
ER -