Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products

Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock

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21 Scopus citations

Abstract

Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL -1. The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.

Original languageEnglish (US)
Pages (from-to)8441-8447
Number of pages7
JournalAnalytical Chemistry
Volume86
Issue number16
DOIs
StatePublished - Aug 19 2014

ASJC Scopus subject areas

  • Analytical Chemistry

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    Hua, X., Yin, W., Shi, H., Li, M., Wang, Y., Wang, H., Ye, Y., Kim, H. J., Gee, S. J., Wang, M., Liu, F., & Hammock, B. D. (2014). Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products. Analytical Chemistry, 86(16), 8441-8447. https://doi.org/10.1021/ac5020657