Development of molecular hybridization technology to evaluate albumin and procollagen mRNA content in baboons and man

F. R. Weiner, M. J. Czaja, M. A. Giambrone, C. H. Wu, G. Y. Wu, Mark A Zern

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have developed the methodology for evaluating the effects of pathophysiological conditions on the molecular mechanisms of hepatic protein synthesis and fibrogenesis in baboons and man. Total RNA was extracted from percutaneous liver biopsies of five baboons who were chronically fed an ethanol-rich liquid diet, their pair-fed controls and from humans with a variety of liver abnormalities. Chronic alcohol administration in baboons with liver fibrosis and normal serum albumin levels increased in vitro protein synthesis as measured by [35S]methionine incorporation, albumin mRNA content and Type I procollagen mRNA content. There was no difference in the β-actin (a constitutive protein) mRNA content. In humans, serum albumin levels correlated with albumin mRNA content as indicated by the intensity of dot blot hybridization and Type I procollagen mRNA levels correlated with the activity of liver fibrosis. The use of RNA-DNA hybridization to investigate procollagen mRNA from human biopsies appears to be a valuable tool for evaluating the potential for collagen synthesis and the future course of liver disease. Besides the use of RNA-DNA hybridization, we describe other methodologies which are useful in delineating the levels of gene expression responsible for hepatic mRNA regulation in normal liver and disease states in man. The use of molecular techniques to evaluate human liver disease provides an opportunity to develop clinically relevant information relevant information while at the same time offering the additional advantage of providing fundamental knowledge about fibrogenesis.

Original languageEnglish (US)
JournalHepatology
Volume7
Issue number1 SUPPL.
StatePublished - 1987
Externally publishedYes

Fingerprint

Procollagen
Papio
Albumins
Technology
Messenger RNA
Liver Diseases
Liver
RNA
Collagen Type I
Serum Albumin
Liver Cirrhosis
Biopsy
Proteins
DNA
Methionine
Ethanol
Collagen
Alcohols
Diet
Gene Expression

ASJC Scopus subject areas

  • Hepatology

Cite this

Weiner, F. R., Czaja, M. J., Giambrone, M. A., Wu, C. H., Wu, G. Y., & Zern, M. A. (1987). Development of molecular hybridization technology to evaluate albumin and procollagen mRNA content in baboons and man. Hepatology, 7(1 SUPPL.).

Development of molecular hybridization technology to evaluate albumin and procollagen mRNA content in baboons and man. / Weiner, F. R.; Czaja, M. J.; Giambrone, M. A.; Wu, C. H.; Wu, G. Y.; Zern, Mark A.

In: Hepatology, Vol. 7, No. 1 SUPPL., 1987.

Research output: Contribution to journalArticle

Weiner, FR, Czaja, MJ, Giambrone, MA, Wu, CH, Wu, GY & Zern, MA 1987, 'Development of molecular hybridization technology to evaluate albumin and procollagen mRNA content in baboons and man', Hepatology, vol. 7, no. 1 SUPPL..
Weiner, F. R. ; Czaja, M. J. ; Giambrone, M. A. ; Wu, C. H. ; Wu, G. Y. ; Zern, Mark A. / Development of molecular hybridization technology to evaluate albumin and procollagen mRNA content in baboons and man. In: Hepatology. 1987 ; Vol. 7, No. 1 SUPPL.
@article{607868f2be144c0aa0b92ab202fa7ab9,
title = "Development of molecular hybridization technology to evaluate albumin and procollagen mRNA content in baboons and man",
abstract = "We have developed the methodology for evaluating the effects of pathophysiological conditions on the molecular mechanisms of hepatic protein synthesis and fibrogenesis in baboons and man. Total RNA was extracted from percutaneous liver biopsies of five baboons who were chronically fed an ethanol-rich liquid diet, their pair-fed controls and from humans with a variety of liver abnormalities. Chronic alcohol administration in baboons with liver fibrosis and normal serum albumin levels increased in vitro protein synthesis as measured by [35S]methionine incorporation, albumin mRNA content and Type I procollagen mRNA content. There was no difference in the β-actin (a constitutive protein) mRNA content. In humans, serum albumin levels correlated with albumin mRNA content as indicated by the intensity of dot blot hybridization and Type I procollagen mRNA levels correlated with the activity of liver fibrosis. The use of RNA-DNA hybridization to investigate procollagen mRNA from human biopsies appears to be a valuable tool for evaluating the potential for collagen synthesis and the future course of liver disease. Besides the use of RNA-DNA hybridization, we describe other methodologies which are useful in delineating the levels of gene expression responsible for hepatic mRNA regulation in normal liver and disease states in man. The use of molecular techniques to evaluate human liver disease provides an opportunity to develop clinically relevant information relevant information while at the same time offering the additional advantage of providing fundamental knowledge about fibrogenesis.",
author = "Weiner, {F. R.} and Czaja, {M. J.} and Giambrone, {M. A.} and Wu, {C. H.} and Wu, {G. Y.} and Zern, {Mark A}",
year = "1987",
language = "English (US)",
volume = "7",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Ltd",
number = "1 SUPPL.",

}

TY - JOUR

T1 - Development of molecular hybridization technology to evaluate albumin and procollagen mRNA content in baboons and man

AU - Weiner, F. R.

AU - Czaja, M. J.

AU - Giambrone, M. A.

AU - Wu, C. H.

AU - Wu, G. Y.

AU - Zern, Mark A

PY - 1987

Y1 - 1987

N2 - We have developed the methodology for evaluating the effects of pathophysiological conditions on the molecular mechanisms of hepatic protein synthesis and fibrogenesis in baboons and man. Total RNA was extracted from percutaneous liver biopsies of five baboons who were chronically fed an ethanol-rich liquid diet, their pair-fed controls and from humans with a variety of liver abnormalities. Chronic alcohol administration in baboons with liver fibrosis and normal serum albumin levels increased in vitro protein synthesis as measured by [35S]methionine incorporation, albumin mRNA content and Type I procollagen mRNA content. There was no difference in the β-actin (a constitutive protein) mRNA content. In humans, serum albumin levels correlated with albumin mRNA content as indicated by the intensity of dot blot hybridization and Type I procollagen mRNA levels correlated with the activity of liver fibrosis. The use of RNA-DNA hybridization to investigate procollagen mRNA from human biopsies appears to be a valuable tool for evaluating the potential for collagen synthesis and the future course of liver disease. Besides the use of RNA-DNA hybridization, we describe other methodologies which are useful in delineating the levels of gene expression responsible for hepatic mRNA regulation in normal liver and disease states in man. The use of molecular techniques to evaluate human liver disease provides an opportunity to develop clinically relevant information relevant information while at the same time offering the additional advantage of providing fundamental knowledge about fibrogenesis.

AB - We have developed the methodology for evaluating the effects of pathophysiological conditions on the molecular mechanisms of hepatic protein synthesis and fibrogenesis in baboons and man. Total RNA was extracted from percutaneous liver biopsies of five baboons who were chronically fed an ethanol-rich liquid diet, their pair-fed controls and from humans with a variety of liver abnormalities. Chronic alcohol administration in baboons with liver fibrosis and normal serum albumin levels increased in vitro protein synthesis as measured by [35S]methionine incorporation, albumin mRNA content and Type I procollagen mRNA content. There was no difference in the β-actin (a constitutive protein) mRNA content. In humans, serum albumin levels correlated with albumin mRNA content as indicated by the intensity of dot blot hybridization and Type I procollagen mRNA levels correlated with the activity of liver fibrosis. The use of RNA-DNA hybridization to investigate procollagen mRNA from human biopsies appears to be a valuable tool for evaluating the potential for collagen synthesis and the future course of liver disease. Besides the use of RNA-DNA hybridization, we describe other methodologies which are useful in delineating the levels of gene expression responsible for hepatic mRNA regulation in normal liver and disease states in man. The use of molecular techniques to evaluate human liver disease provides an opportunity to develop clinically relevant information relevant information while at the same time offering the additional advantage of providing fundamental knowledge about fibrogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0023134264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023134264&partnerID=8YFLogxK

M3 - Article

C2 - 3804213

AN - SCOPUS:0023134264

VL - 7

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 1 SUPPL.

ER -