Development of in vitro fertilized oocytes from pregnant and nonpregnant cows in oviductal epithelial and cumulus cell co-culture systems

E. Behboodi, G. B. Anderson, Robert Bondurant

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The objectives of this study were 1) to measure cleavage, blastocyst formation, and blastocyst hatching after in vitro maturation (IVM), fertilization (IVF) and culture (IVC) of oocytes aspirated from pregnant versus nonpregnant cows, and 2) to compare embryo development in co-culture with bovine oviductal epithelial cells versus cumulus cells. No differences in cleavage (38 versus 40%), blastocyst formation (13 versus 13%), or blastocyst hatching (53 versus 51%) were observed for in vitro-matured, fertilized, and cultured oocytes from pregnant versus nonpregnant cows, respectively (P > 0.05), indicating that nonpregnant and early-pregnant cows are equally acceptable donors of oocytes for IVM/IVF/IVC procedures. Cleavage (36 versus 40%), blastocyst formation (11 versus 12%), and blastocyst hatching (50 versus 55%) were not different for embryos co-cultured with oviductal epithelial cells versus cumulus cells (P > 0.05). Thus, equivalent embryo development can be obtained with co-culture systems commonly used for in vitro-derived bovine embryos. These results help to define variables that affect comparison of results across laboratories and that are relevant to the practical application of IVM/IVF/IVC procedures to cattle.

Original languageEnglish (US)
Pages (from-to)1077-1084
Number of pages8
JournalTheriogenology
Volume38
Issue number6
DOIs
StatePublished - Jan 1 1992

Fingerprint

Cumulus Cells
Blastocyst
coculture
Coculture Techniques
blastocyst
Oocytes
oocytes
Cell Culture Techniques
Epithelial Cells
cows
fertilization (reproduction)
hatching
cells
Fertilization
Embryonic Development
cattle
embryo (animal)
epithelial cells
embryogenesis
Embryonic Structures

Keywords

  • bovine
  • embryos
  • in vitro fertilization
  • in vitro maturation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Development of in vitro fertilized oocytes from pregnant and nonpregnant cows in oviductal epithelial and cumulus cell co-culture systems. / Behboodi, E.; Anderson, G. B.; Bondurant, Robert.

In: Theriogenology, Vol. 38, No. 6, 01.01.1992, p. 1077-1084.

Research output: Contribution to journalArticle

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