Abstract
Intravenous (i.v.) infusion of excessive energy has been associated with hepatic steatosis. The time course of liver lipid accumulation was examined during 6 days of i.v. hyperalimentation with fat-free infusate. Adult male rats with indwelling superior vena cava cannulas received a dextrose-amino acid infusate for 0, 1/2 , 1, 2, 4 or 6 days to provide 146% of nonprotein energy requirement [≃350 nonprotein kcal/kcal/(Kg · day)] and 335% of nitrogen requirement [ ≃ 2.7 g amino nitrogen (kg · day)]. Significant hepatomegaly was apparent by day 1/2 . Initially, glycogen deposition accounted for the liver enlargement, but after day 2, liver glycogen was declining and liver lipid was increasing. By day 4, liver lipid had increased fourfold and was the major contributor to hepatomegaly. Concurrent with fatty liver metamorphosis, hepatic essential fatty acid deficiency (EFAD) developed by day 4; liver linoleic acid levels had dropped from 20 to 1% of total fatty acids, and liver triene:tetraene ratio was 0.68. Similar changes in hepatic phospholipid fatty acids were observed. Enhanced lipogenesis and impaired lipid transport is known to accompany EFAD and may underlie the observed steatosis. A doubling of plasma cholesterol levels was also associated with steatosis. The mechanism leading to this increase in plasma cholesterol warrants further investigation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1807-1815 |
| Number of pages | 9 |
| Journal | Journal of Nutrition |
| Volume | 114 |
| Issue number | 10 |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
Cite this
Development of hepatic steatosis and essential fatty acid deficiency in rats with hypercaloric, fat-free parenteral nutrition. / Keim, N. L.; Mares-Perlman, J. A.
In: Journal of Nutrition, Vol. 114, No. 10, 1984, p. 1807-1815.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of hepatic steatosis and essential fatty acid deficiency in rats with hypercaloric, fat-free parenteral nutrition
AU - Keim, N. L.
AU - Mares-Perlman, J. A.
PY - 1984
Y1 - 1984
N2 - Intravenous (i.v.) infusion of excessive energy has been associated with hepatic steatosis. The time course of liver lipid accumulation was examined during 6 days of i.v. hyperalimentation with fat-free infusate. Adult male rats with indwelling superior vena cava cannulas received a dextrose-amino acid infusate for 0, 1/2 , 1, 2, 4 or 6 days to provide 146% of nonprotein energy requirement [≃350 nonprotein kcal/kcal/(Kg · day)] and 335% of nitrogen requirement [ ≃ 2.7 g amino nitrogen (kg · day)]. Significant hepatomegaly was apparent by day 1/2 . Initially, glycogen deposition accounted for the liver enlargement, but after day 2, liver glycogen was declining and liver lipid was increasing. By day 4, liver lipid had increased fourfold and was the major contributor to hepatomegaly. Concurrent with fatty liver metamorphosis, hepatic essential fatty acid deficiency (EFAD) developed by day 4; liver linoleic acid levels had dropped from 20 to 1% of total fatty acids, and liver triene:tetraene ratio was 0.68. Similar changes in hepatic phospholipid fatty acids were observed. Enhanced lipogenesis and impaired lipid transport is known to accompany EFAD and may underlie the observed steatosis. A doubling of plasma cholesterol levels was also associated with steatosis. The mechanism leading to this increase in plasma cholesterol warrants further investigation.
AB - Intravenous (i.v.) infusion of excessive energy has been associated with hepatic steatosis. The time course of liver lipid accumulation was examined during 6 days of i.v. hyperalimentation with fat-free infusate. Adult male rats with indwelling superior vena cava cannulas received a dextrose-amino acid infusate for 0, 1/2 , 1, 2, 4 or 6 days to provide 146% of nonprotein energy requirement [≃350 nonprotein kcal/kcal/(Kg · day)] and 335% of nitrogen requirement [ ≃ 2.7 g amino nitrogen (kg · day)]. Significant hepatomegaly was apparent by day 1/2 . Initially, glycogen deposition accounted for the liver enlargement, but after day 2, liver glycogen was declining and liver lipid was increasing. By day 4, liver lipid had increased fourfold and was the major contributor to hepatomegaly. Concurrent with fatty liver metamorphosis, hepatic essential fatty acid deficiency (EFAD) developed by day 4; liver linoleic acid levels had dropped from 20 to 1% of total fatty acids, and liver triene:tetraene ratio was 0.68. Similar changes in hepatic phospholipid fatty acids were observed. Enhanced lipogenesis and impaired lipid transport is known to accompany EFAD and may underlie the observed steatosis. A doubling of plasma cholesterol levels was also associated with steatosis. The mechanism leading to this increase in plasma cholesterol warrants further investigation.
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M3 - Article
VL - 114
SP - 1807
EP - 1815
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 10
ER -