Development of ELISAs for the class-specific determination of organophosphorus pesticides

Yuan Zhe Piao, Yoo Jung Kim, Young A H Kim, Hye Sung Lee, Bruce D. Hammock, Yong Tae Lee

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Enzyme-linked immunosorbent assays (ELISAs) for the class-specific determination of organophosphorus (OP) pesticides were developed from monoclonal antibodies raised against haptens with the functional group common to OP pesticides. To develop antigen-coated, indirect, competitive ELISAs, four haptens with different spacer arm structures were used to prepare antibodies, while eight haptens were tested for use as coating antigens. A total of 32 ELISAs were developed with one selected as the most suitable one based on average IC50 and % CV values. The chosen ELISA showed class-selective response to O,O-diethyl phosphorothioate and phosphorodithioate OP pesticides with negligible cross-reactivity to other types of pesticides. Average IC50 and % CV values of this ELISA for the 12 OP pesticides were 89 ng/mL and 96%, respectively. Compared to ELISAs previously developed with the same objective, the current ELISA demonstrates better sensitivity based on much lower mean IC50 values in addition to improved class-selective determination based on considerably lower % CV values as well as precise discrimination against other types of pesticides.

Original languageEnglish (US)
Pages (from-to)10004-10013
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number21
DOIs
StatePublished - Nov 11 2009

Keywords

  • Class-specific determination
  • Enzyme-linked immunosorbent assay
  • Immunoassay
  • Organophosphorus pesticide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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    Piao, Y. Z., Kim, Y. J., Kim, Y. A. H., Lee, H. S., Hammock, B. D., & Lee, Y. T. (2009). Development of ELISAs for the class-specific determination of organophosphorus pesticides. Journal of Agricultural and Food Chemistry, 57(21), 10004-10013. https://doi.org/10.1021/jf901998y