Abstract
In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1471-1476 |
| Number of pages | 6 |
| Journal | Chemosphere |
| Volume | 46 |
| Issue number | 9-10 |
| DOIs | |
| State | Published - 2002 |
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Keywords
- GC/MS
- Immunoassay
- PCDDs
- PCDFs
- TCDD
- Validation
ASJC Scopus subject areas
- Environmental Chemistry
- Environmental Science(all)
Cite this
Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. / Sugawara, Yukio; Saito, Koichi; Ogawa, Masahiko; Kobayashi, Susumu; Shan, Guomin; Sanborn, James R.; Hammock, Bruce D.; Nakazawa, Hiroyuki; Matsuki, Yasuhiko.
In: Chemosphere, Vol. 46, No. 9-10, 2002, p. 1471-1476.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk
AU - Sugawara, Yukio
AU - Saito, Koichi
AU - Ogawa, Masahiko
AU - Kobayashi, Susumu
AU - Shan, Guomin
AU - Sanborn, James R.
AU - Hammock, Bruce D.
AU - Nakazawa, Hiroyuki
AU - Matsuki, Yasuhiko
PY - 2002
Y1 - 2002
N2 - In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.
AB - In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.
KW - GC/MS
KW - Immunoassay
KW - PCDDs
KW - PCDFs
KW - TCDD
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=0036124342&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036124342&partnerID=8YFLogxK
U2 - 10.1016/S0045-6535(01)00267-3
DO - 10.1016/S0045-6535(01)00267-3
M3 - Article
C2 - 12002478
AN - SCOPUS:0036124342
VL - 46
SP - 1471
EP - 1476
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 9-10
ER -