Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk

Yukio Sugawara; Koichi Saito; Masahiko Ogawa; Susumu Kobayashi; Guomin Shan; James R. Sanborn; Bruce D. Hammock; Hiroyuki Nakazawa; Yasuhiko Matsuki

doi:10.1016/S0045-6535(01)00267-3

Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk

Yukio Sugawara, Koichi Saito, Masahiko Ogawa, Susumu Kobayashi, Guomin Shan, James R. Sanborn, Bruce D. Hammock, Hiroyuki Nakazawa, Yasuhiko Matsuki

Entomology

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20 Scopus citations

Abstract

In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.

Original language	English (US)
Pages (from-to)	1471-1476
Number of pages	6
Journal	Chemosphere
Volume	46
Issue number	9-10
DOIs	https://doi.org/10.1016/S0045-6535(01)00267-3
State	Published - 2002

Keywords

GC/MS
Immunoassay
PCDDs
PCDFs
TCDD
Validation

ASJC Scopus subject areas

Environmental Chemistry
Environmental Science(all)

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Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. / Sugawara, Yukio; Saito, Koichi; Ogawa, Masahiko; Kobayashi, Susumu; Shan, Guomin; Sanborn, James R.; Hammock, Bruce D.; Nakazawa, Hiroyuki; Matsuki, Yasuhiko.

In: Chemosphere, Vol. 46, No. 9-10, 2002, p. 1471-1476.

Research output: Contribution to journal › Article › peer-review

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AU - Sugawara, Yukio

AU - Saito, Koichi

AU - Ogawa, Masahiko

AU - Kobayashi, Susumu

AU - Shan, Guomin

AU - Sanborn, James R.

AU - Hammock, Bruce D.

AU - Nakazawa, Hiroyuki

AU - Matsuki, Yasuhiko

PY - 2002

Y1 - 2002

N2 - In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.

AB - In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.

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KW - Immunoassay

KW - PCDDs

KW - PCDFs

KW - TCDD

KW - Validation

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Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk

Abstract

Keywords

ASJC Scopus subject areas

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