Abstract
In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.
Original language | English (US) |
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Pages (from-to) | 1471-1476 |
Number of pages | 6 |
Journal | Chemosphere |
Volume | 46 |
Issue number | 9-10 |
DOIs | |
State | Published - 2002 |
Keywords
- GC/MS
- Immunoassay
- PCDDs
- PCDFs
- TCDD
- Validation
ASJC Scopus subject areas
- Environmental Chemistry
- Environmental Science(all)