Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk

Yukio Sugawara, Koichi Saito, Masahiko Ogawa, Susumu Kobayashi, Guomin Shan, James R. Sanborn, Bruce D. Hammock, Hiroyuki Nakazawa, Yasuhiko Matsuki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.

Original languageEnglish (US)
Pages (from-to)1471-1476
Number of pages6
JournalChemosphere
Volume46
Issue number9-10
DOIs
StatePublished - 2002

Fingerprint

Immunosorbents
Dioxins
Human Milk
dioxin
milk
Toxicity
Assays
Enzymes
Enzyme-Linked Immunosorbent Assay
assay
enzyme
toxicity
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
gas chromatography
mass spectrometry
Environmental Monitoring
immunoassay
Immunoassay

Keywords

  • GC/MS
  • Immunoassay
  • PCDDs
  • PCDFs
  • TCDD
  • Validation

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Sugawara, Y., Saito, K., Ogawa, M., Kobayashi, S., Shan, G., Sanborn, J. R., ... Matsuki, Y. (2002). Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. Chemosphere, 46(9-10), 1471-1476. https://doi.org/10.1016/S0045-6535(01)00267-3

Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. / Sugawara, Yukio; Saito, Koichi; Ogawa, Masahiko; Kobayashi, Susumu; Shan, Guomin; Sanborn, James R.; Hammock, Bruce D.; Nakazawa, Hiroyuki; Matsuki, Yasuhiko.

In: Chemosphere, Vol. 46, No. 9-10, 2002, p. 1471-1476.

Research output: Contribution to journalArticle

Sugawara, Y, Saito, K, Ogawa, M, Kobayashi, S, Shan, G, Sanborn, JR, Hammock, BD, Nakazawa, H & Matsuki, Y 2002, 'Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk', Chemosphere, vol. 46, no. 9-10, pp. 1471-1476. https://doi.org/10.1016/S0045-6535(01)00267-3
Sugawara, Yukio ; Saito, Koichi ; Ogawa, Masahiko ; Kobayashi, Susumu ; Shan, Guomin ; Sanborn, James R. ; Hammock, Bruce D. ; Nakazawa, Hiroyuki ; Matsuki, Yasuhiko. / Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. In: Chemosphere. 2002 ; Vol. 46, No. 9-10. pp. 1471-1476.
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