Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk

Yukio Sugawara, Koichi Saito, Masahiko Ogawa, Susumu Kobayashi, Guomin Shan, James R. Sanborn, Bruce D. Hammock, Hiroyuki Nakazawa, Yasuhiko Matsuki

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.

Original languageEnglish (US)
Pages (from-to)1471-1476
Number of pages6
JournalChemosphere
Volume46
Issue number9-10
DOIs
StatePublished - 2002

    Fingerprint

Keywords

  • GC/MS
  • Immunoassay
  • PCDDs
  • PCDFs
  • TCDD
  • Validation

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Sugawara, Y., Saito, K., Ogawa, M., Kobayashi, S., Shan, G., Sanborn, J. R., Hammock, B. D., Nakazawa, H., & Matsuki, Y. (2002). Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. Chemosphere, 46(9-10), 1471-1476. https://doi.org/10.1016/S0045-6535(01)00267-3