Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk

Yukio Sugawara; Koichi Saito; Masahiko Ogawa; Susumu Kobayashi; Guomin Shan; James R. Sanborn; Bruce D. Hammock; Hiroyuki Nakazawa; Yasuhiko Matsuki

doi:10.1016/S0045-6535(01)00267-3

Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk

Yukio Sugawara, Koichi Saito, Masahiko Ogawa, Susumu Kobayashi, Guomin Shan, James R. Sanborn, Bruce D. Hammock, Hiroyuki Nakazawa, Yasuhiko Matsuki

Entomology

Research output: Contribution to journal › Article

18 Scopus citations

Abstract

In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r = 0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring.

Original language	English (US)
Pages (from-to)	1471-1476
Number of pages	6
Journal	Chemosphere
Volume	46
Issue number	9-10
DOIs	https://doi.org/10.1016/S0045-6535(01)00267-3
State	Published - 2002

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Keywords

GC/MS
Immunoassay
PCDDs
PCDFs
TCDD
Validation

ASJC Scopus subject areas

Environmental Chemistry
Environmental Science(all)

Cite this

Sugawara, Y., Saito, K., Ogawa, M., Kobayashi, S., Shan, G., Sanborn, J. R., Hammock, B. D., Nakazawa, H., & Matsuki, Y. (2002). Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. Chemosphere, 46(9-10), 1471-1476. https://doi.org/10.1016/S0045-6535(01)00267-3

Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay - Assay validation for human milk. / Sugawara, Yukio; Saito, Koichi; Ogawa, Masahiko; Kobayashi, Susumu; Shan, Guomin; Sanborn, James R.; Hammock, Bruce D.; Nakazawa, Hiroyuki; Matsuki, Yasuhiko.

In: Chemosphere, Vol. 46, No. 9-10, 2002, p. 1471-1476.

Research output: Contribution to journal › Article

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10.1016/S0045-6535(01)00267-3