Development of bovine and porcine embryonic teratomas in athymic mice

G. B. Anderson, Robert Bondurant, L. Goff, J. Groff, A. L. Moyer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Inner cell masses (ICM) and embryonic discs from bovine and porcine blastocysts of various ages were transplanted under the kidney capsule of athymic (nude) mice to evaluate growth of teratocarcinomas containing both differentiated tissues and undifferentiated stem cells. Inner cell masses were isolated immunosurgically from Day 8, Day 9 and Day 10 porcine blastocysts and from Day 8, Day 10 and Day 12 bovine blastocysts. Embryonic discs were mechanically dissected from Day 11 and Day 12 porcine embryos and from Day 14 bovine embryos. Day 6 egg cylinders were dissected from BALB/C embryos and from hybrid embryos of a cross between BALB/C and an outbred strain of mouse. Two to four ICM, embryonic discs or egg cylinders were transplanted under the kidney capsule of each athymic host. After 8 weeks, graft hosts were killed and their tumors removed, fixed and prepared for histological and immunohistochemical examination. Embryonic teratomas developed at high frequency from murine egg cylinders and from Day 11 and Day 12 porcine and Day 14 bovine embryos. Tumors were observed only infrequently from younger bovine and porcine blastocysts. Murine embryonic tumors were composed of numerous differentiated cell types of ectodermal, mesodermal and endodermal origins, but representation of the three embryonic germ layers was somewhat more restricted in bovine and porcine embryonic tumors. No undifferentiated stem cells were detected in tumors of any of the three species. These results demonstrate that teratomas will develop from bovine and porcine embryos when grafted to an immunocompromised host, but the presence of undifferentiated teratocarcinoma stem cells from these species has yet to be achieved.

Original languageEnglish (US)
Pages (from-to)231-240
Number of pages10
JournalAnimal Reproduction Science
Volume45
Issue number3
DOIs
StatePublished - Dec 16 1996

Fingerprint

Teratoma
Nude Mice
Swine
embryo (animal)
Embryonic Structures
Blastocyst
swine
cattle
mice
blastocyst
neoplasms
Ovum
stem cells
Neoplasms
Capsules
Stem Cells
kidneys
cells
Teratocarcinoma
immunocompromised population

Keywords

  • Cattle embryology
  • EC cells
  • Pig-embryology
  • Teratocarcinomas
  • Teratomas

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

Cite this

Development of bovine and porcine embryonic teratomas in athymic mice. / Anderson, G. B.; Bondurant, Robert; Goff, L.; Groff, J.; Moyer, A. L.

In: Animal Reproduction Science, Vol. 45, No. 3, 16.12.1996, p. 231-240.

Research output: Contribution to journalArticle

Anderson, G. B. ; Bondurant, Robert ; Goff, L. ; Groff, J. ; Moyer, A. L. / Development of bovine and porcine embryonic teratomas in athymic mice. In: Animal Reproduction Science. 1996 ; Vol. 45, No. 3. pp. 231-240.
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