Development of antibodies against hydroxyatrazine and hydroxysimazine: Application to environmental samples

Anne D. Lucas, Hassan K M Bekheit, Marvin H. Goodrow, A. Daniel Jones, Seth Kullman, Fumio Matsumura, James E. Woodrow, James N. Seiber, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 % of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.

Original languageEnglish (US)
Pages (from-to)1523-1529
Number of pages7
JournalJournal of Agricultural and Food Chemistry®
Issue number9
StatePublished - 1993

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


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