Development of antibodies against hydroxyatrazine and hydroxysimazine: Application to environmental samples

Anne D. Lucas, Hassan K M Bekheit, Marvin H. Goodrow, A. Daniel Jones, Seth Kullman, Fumio Matsumura, James E. Woodrow, James N. Seiber, Bruce D. Hammock

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 % of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.

Original languageEnglish (US)
Pages (from-to)1523-1529
Number of pages7
JournalJournal of Agricultural and Food Chemistry®
Volume41
Issue number9
StatePublished - 1993

Fingerprint

Atrazine
atrazine
Immunosorbents
antibodies
Antibodies
Assays
Enzyme-Linked Immunosorbent Assay
horse manure
enzyme-linked immunosorbent assay
Environmental Biodegradation
Manure
Bioremediation
Manures
bioremediation
Enzymes
sampling
Horses
Phanerochaete
Phanerochaete chrysosporium
drums (equipment)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Lucas, A. D., Bekheit, H. K. M., Goodrow, M. H., Jones, A. D., Kullman, S., Matsumura, F., ... Hammock, B. D. (1993). Development of antibodies against hydroxyatrazine and hydroxysimazine: Application to environmental samples. Journal of Agricultural and Food Chemistry®, 41(9), 1523-1529.

Development of antibodies against hydroxyatrazine and hydroxysimazine : Application to environmental samples. / Lucas, Anne D.; Bekheit, Hassan K M; Goodrow, Marvin H.; Jones, A. Daniel; Kullman, Seth; Matsumura, Fumio; Woodrow, James E.; Seiber, James N.; Hammock, Bruce D.

In: Journal of Agricultural and Food Chemistry®, Vol. 41, No. 9, 1993, p. 1523-1529.

Research output: Contribution to journalArticle

Lucas, AD, Bekheit, HKM, Goodrow, MH, Jones, AD, Kullman, S, Matsumura, F, Woodrow, JE, Seiber, JN & Hammock, BD 1993, 'Development of antibodies against hydroxyatrazine and hydroxysimazine: Application to environmental samples', Journal of Agricultural and Food Chemistry®, vol. 41, no. 9, pp. 1523-1529.
Lucas, Anne D. ; Bekheit, Hassan K M ; Goodrow, Marvin H. ; Jones, A. Daniel ; Kullman, Seth ; Matsumura, Fumio ; Woodrow, James E. ; Seiber, James N. ; Hammock, Bruce D. / Development of antibodies against hydroxyatrazine and hydroxysimazine : Application to environmental samples. In: Journal of Agricultural and Food Chemistry®. 1993 ; Vol. 41, No. 9. pp. 1523-1529.
@article{3745c77400674248b63dfb126e652645,
title = "Development of antibodies against hydroxyatrazine and hydroxysimazine: Application to environmental samples",
abstract = "An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 {\%} of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.",
author = "Lucas, {Anne D.} and Bekheit, {Hassan K M} and Goodrow, {Marvin H.} and Jones, {A. Daniel} and Seth Kullman and Fumio Matsumura and Woodrow, {James E.} and Seiber, {James N.} and Hammock, {Bruce D.}",
year = "1993",
language = "English (US)",
volume = "41",
pages = "1523--1529",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Development of antibodies against hydroxyatrazine and hydroxysimazine

T2 - Application to environmental samples

AU - Lucas, Anne D.

AU - Bekheit, Hassan K M

AU - Goodrow, Marvin H.

AU - Jones, A. Daniel

AU - Kullman, Seth

AU - Matsumura, Fumio

AU - Woodrow, James E.

AU - Seiber, James N.

AU - Hammock, Bruce D.

PY - 1993

Y1 - 1993

N2 - An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 % of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.

AB - An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 % of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.

UR - http://www.scopus.com/inward/record.url?scp=0012436966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0012436966&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0012436966

VL - 41

SP - 1523

EP - 1529

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 9

ER -