Abstract
An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 % of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1523-1529 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry® |
| Volume | 41 |
| Issue number | 9 |
| State | Published - 1993 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
Development of antibodies against hydroxyatrazine and hydroxysimazine : Application to environmental samples. / Lucas, Anne D.; Bekheit, Hassan K M; Goodrow, Marvin H.; Jones, A. Daniel; Kullman, Seth; Matsumura, Fumio; Woodrow, James E.; Seiber, James N.; Hammock, Bruce D.
In: Journal of Agricultural and Food Chemistry®, Vol. 41, No. 9, 1993, p. 1523-1529.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of antibodies against hydroxyatrazine and hydroxysimazine
T2 - Application to environmental samples
AU - Lucas, Anne D.
AU - Bekheit, Hassan K M
AU - Goodrow, Marvin H.
AU - Jones, A. Daniel
AU - Kullman, Seth
AU - Matsumura, Fumio
AU - Woodrow, James E.
AU - Seiber, James N.
AU - Hammock, Bruce D.
PY - 1993
Y1 - 1993
N2 - An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 % of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.
AB - An enzyme-linked immunosorbent assay (ELISA) selective for hydroxyatrazine and hydroxysimazine was developed an a means of monitoring environmental and microbial degradation of atrazine to hydroxyatrazine. This ELISA was tolerant to solvents, salts, and pH changes and functioned well in a series of matrices (water, soil, horse manure, urine, and fungal extracts). In preliminary bioremediation studies conducted in a drum impervious to UV light, the microbes endogenous to horse manure converted approximately 1 % of the total initial amount of atrazine to hydroxyatrazine. Also, a UV-resistant strain of white rot fungus (Phanerochaete chrysosporium) was tested for its ability to degrade atrazine. Using the ELISA described here, approximately half of the time zero amount of atrazine (2 μg/35-mm Petri dish) was gone in 1 day and essentially all of the atrazine was converted to hydroxyatrazine, primarily due to UV irradiation. This ELISA provided a valuable method to quantify hydroxyatrazine and hydroxysimazine in a series of traditionally difficult matrices for the investigation of the bioremediation of atrazine.
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UR - http://www.scopus.com/inward/citedby.url?scp=0012436966&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0012436966
VL - 41
SP - 1523
EP - 1529
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 9
ER -