Development of an interleukin-2 receptor expression assay and its use in evaluation of cellular immune responses in bottlenose dolphin (Tursiops truncatus).

K. L. Erickson, L. DiMolfetto-Landon, R. S. Wells, T. Reidarson, Jeffrey L Stott, D. A. Ferrick

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We describe optimization of a peripheral blood mononuclear leukocyte proliferation assay and development of an interleukin-2 receptor (IL-2R) expression assay for bottlenose dolphins (Tursiops truncatus). Peripheral blood mononuclear leukocytes obtained from both Sea World (February 1993) and the Naval Command Control and Ocean Surveillance Center (March 1993) (San Diego, California, USA) were stimulated with the mitogens concanavalin A (ConA) and phytohemagglutinin (PHA) and evaluated for optimum proliferation and IL-2R expression. Based on these optimization assays, standard conditions were established and used to assess immune function in a population of apparently healthy, free-ranging bottlenose dolphins from Sarasota Bay, Florida (USA) in June 1993. A positive correlation was observed between proliferation assays using ConA and PHA as the stimulants. However, IL-2R expression induced by both mitogens differed significantly.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalJournal of Wildlife Diseases
Volume31
Issue number2
StatePublished - Apr 1995

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Bottle-Nosed Dolphin
Interleukin-2 Receptors
Tursiops truncatus
dolphin
interleukin-2
immune response
Cellular Immunity
cell-mediated immunity
Mononuclear Leukocytes
assay
Phytohemagglutinins
Concanavalin A
Mitogens
Oceans and Seas
receptors
phytohemagglutinin
assays
concanavalin A
mononuclear leukocytes
blood

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Development of an interleukin-2 receptor expression assay and its use in evaluation of cellular immune responses in bottlenose dolphin (Tursiops truncatus). / Erickson, K. L.; DiMolfetto-Landon, L.; Wells, R. S.; Reidarson, T.; Stott, Jeffrey L; Ferrick, D. A.

In: Journal of Wildlife Diseases, Vol. 31, No. 2, 04.1995, p. 142-149.

Research output: Contribution to journalArticle

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AU - Stott, Jeffrey L

AU - Ferrick, D. A.

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