Development of an Immunoassay for the Detection of the Phenylpyrazole Insecticide Fipronil

Natalia Vasylieva, Ki Chang Ahn, Bogdan Barnych, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Phenylpyrazole insecticides such as fipronil have been used as replacements for organophosphates. The wide application of fipronil raises concern about environmental contamination and risk for fish, birds, and other nontargeted beings as well as human health. A sensitive, competitive indirect heterologous enzyme-linked immunosorbent assay (ELISA) was developed. Antibodies with different specificities to fipronil and its metabolites were produced. Two ELISAs having IC<inf>50</inf> values of 0.58 ± 0.06 and 2.6 ± 0.4 ng/mL were developed. Design of different haptens and coating antigens resulted in two assays with distinct cross-reactivity patterns for structurally related compounds: 96, 38, and 101% versus 39, 1.4, and 25% for fipronil-sulfide, fipronil-detrifluoromethylsulfonyl, and fipronil-desulfinyl, respectively. Performance of the immunoassays was demonstrated by a recovery study from spiked water and human serum and urine matrices, giving recovery values in the range of 85-111% for different concentrations. The assays demonstrated good correlation in fipronil recovery with conventional LC-MS/MS analysis. The generic assay 2265 has the sensitivity to measure fipronil and its analogs in serum at levels relevant for exposure monitoring. The assays were used to analyze human urine samples obtained from exposure studies and serum samples from rats treated with a fipronil-containing diet.

Original languageEnglish (US)
Pages (from-to)10038-10047
Number of pages10
JournalEnvironmental Science and Technology
Issue number16
StatePublished - Jul 21 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry


Dive into the research topics of 'Development of an Immunoassay for the Detection of the Phenylpyrazole Insecticide Fipronil'. Together they form a unique fingerprint.

Cite this