Development of an equine coronavirus–specific enzyme-linked immunosorbent assay to determine serologic responses in naturally infected horses

Lotte J. Kooijman, Samantha M. Mapes, Nicola Pusterla

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Equine coronavirus (EqCoV) infection has been documented in most reports through quantitative qPCR analysis of feces and viral genome sequencing. Although qPCR is used to detect antigen during the acute disease phase, there is no equine-specific antibody test available to study EqCoV seroprevalence in various horse populations. We developed an enzyme-linked immunosorbent assay (ELISA) targeting antibodies to the spike (S) protein of EqCoV and validated its use, using acute and convalescent sera from 83 adult horses involved in 6 outbreaks. The EqCoV S protein–based ELISA was able to reliably detect antibodies to EqCoV in naturally infected horses. The greatest seroconversion rate was observed in horses with clinical signs compatible with EqCoV infection and EqCoV qPCR detection in feces. The EqCoV S protein–based ELISA could be used effectively for seroepidemiologic studies in order to better characterize the overall infection rate of EqCoV in various horse populations.

Original languageEnglish (US)
Pages (from-to)414-418
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume28
Issue number4
DOIs
StatePublished - Jul 1 2016

Fingerprint

Horses
Enzyme-Linked Immunosorbent Assay
Coronavirinae
enzyme-linked immunosorbent assay
horses
Coronavirus
Coronavirus Infections
Seroepidemiologic Studies
Feces
antibodies
Antibodies
feces
infection
acute course
Viral Genome
Protein S
seroconversion
Acute Disease
serological surveys
seroprevalence

Keywords

  • Enzyme-linked immunosorbent assay
  • equine coronavirus
  • natural infection
  • seroprevalence

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Development of an equine coronavirus–specific enzyme-linked immunosorbent assay to determine serologic responses in naturally infected horses. / Kooijman, Lotte J.; Mapes, Samantha M.; Pusterla, Nicola.

In: Journal of Veterinary Diagnostic Investigation, Vol. 28, No. 4, 01.07.2016, p. 414-418.

Research output: Contribution to journalArticle

@article{e8fe3bf477e147bbbadff7af59d3eb94,
title = "Development of an equine coronavirus–specific enzyme-linked immunosorbent assay to determine serologic responses in naturally infected horses",
abstract = "Equine coronavirus (EqCoV) infection has been documented in most reports through quantitative qPCR analysis of feces and viral genome sequencing. Although qPCR is used to detect antigen during the acute disease phase, there is no equine-specific antibody test available to study EqCoV seroprevalence in various horse populations. We developed an enzyme-linked immunosorbent assay (ELISA) targeting antibodies to the spike (S) protein of EqCoV and validated its use, using acute and convalescent sera from 83 adult horses involved in 6 outbreaks. The EqCoV S protein–based ELISA was able to reliably detect antibodies to EqCoV in naturally infected horses. The greatest seroconversion rate was observed in horses with clinical signs compatible with EqCoV infection and EqCoV qPCR detection in feces. The EqCoV S protein–based ELISA could be used effectively for seroepidemiologic studies in order to better characterize the overall infection rate of EqCoV in various horse populations.",
keywords = "Enzyme-linked immunosorbent assay, equine coronavirus, natural infection, seroprevalence",
author = "Kooijman, {Lotte J.} and Mapes, {Samantha M.} and Nicola Pusterla",
year = "2016",
month = "7",
day = "1",
doi = "10.1177/1040638716649643",
language = "English (US)",
volume = "28",
pages = "414--418",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "4",

}

TY - JOUR

T1 - Development of an equine coronavirus–specific enzyme-linked immunosorbent assay to determine serologic responses in naturally infected horses

AU - Kooijman, Lotte J.

AU - Mapes, Samantha M.

AU - Pusterla, Nicola

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Equine coronavirus (EqCoV) infection has been documented in most reports through quantitative qPCR analysis of feces and viral genome sequencing. Although qPCR is used to detect antigen during the acute disease phase, there is no equine-specific antibody test available to study EqCoV seroprevalence in various horse populations. We developed an enzyme-linked immunosorbent assay (ELISA) targeting antibodies to the spike (S) protein of EqCoV and validated its use, using acute and convalescent sera from 83 adult horses involved in 6 outbreaks. The EqCoV S protein–based ELISA was able to reliably detect antibodies to EqCoV in naturally infected horses. The greatest seroconversion rate was observed in horses with clinical signs compatible with EqCoV infection and EqCoV qPCR detection in feces. The EqCoV S protein–based ELISA could be used effectively for seroepidemiologic studies in order to better characterize the overall infection rate of EqCoV in various horse populations.

AB - Equine coronavirus (EqCoV) infection has been documented in most reports through quantitative qPCR analysis of feces and viral genome sequencing. Although qPCR is used to detect antigen during the acute disease phase, there is no equine-specific antibody test available to study EqCoV seroprevalence in various horse populations. We developed an enzyme-linked immunosorbent assay (ELISA) targeting antibodies to the spike (S) protein of EqCoV and validated its use, using acute and convalescent sera from 83 adult horses involved in 6 outbreaks. The EqCoV S protein–based ELISA was able to reliably detect antibodies to EqCoV in naturally infected horses. The greatest seroconversion rate was observed in horses with clinical signs compatible with EqCoV infection and EqCoV qPCR detection in feces. The EqCoV S protein–based ELISA could be used effectively for seroepidemiologic studies in order to better characterize the overall infection rate of EqCoV in various horse populations.

KW - Enzyme-linked immunosorbent assay

KW - equine coronavirus

KW - natural infection

KW - seroprevalence

UR - http://www.scopus.com/inward/record.url?scp=84978245437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978245437&partnerID=8YFLogxK

U2 - 10.1177/1040638716649643

DO - 10.1177/1040638716649643

M3 - Article

VL - 28

SP - 414

EP - 418

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 4

ER -