Development of an Enzyme-Linked Immunosorbent Assay for the Pyrethroid Cypermethrin

Hu Jang Lee, Guomin Shan, Ki Chang Ahn, Eun Kee Park, Takaho Watanabe, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of cypermethrin was developed. Two haptens, the trans- and cis-isomers of 3-[(±)-cyano-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropnecarbonyloxy] methyl]phenoxyacetic acid, were conjugated with thyroglobulin as immunogens. Four antisera were generated and screened against six different coating antigens. The assay that was the most sensitive for cypermethrin was optimized and characterized. The IC50 for cypermethrin was 13.5 ± 4.3 μg/L, and the lower detection limit (LDL) was 1.3 ± 0.5 μg/L. This ELISA had relatively low cross-reactivities with other major pyrethroids, such as deltamethrin, phenothrin, resmethrin, fluvalinate, and permethrin. Methanol was found to be the best organic cosolvent for this ELISA, with an optimal sensitivity observed at a concentration of 40% (v/v). The assay parameters were unchanged at pH values between 5.0 and 8.0, whereas higher ionic strengths strongly suppressed the absorbances. To increase the sensitivity of the overall method, a C18 sorbent-based solid-phase extraction was applied to various domestic and environmental water samples. The water samples, fortified with cypermethrin, were analyzed according to this method. Good recoveries and correlation with spike levels were observed.

Original languageEnglish (US)
Pages (from-to)1039-1043
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number5
StatePublished - Mar 10 2004


  • Cypermethrin
  • Environmental monitoring
  • Pyrethroid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


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