Development of an enzyme-linked immunosorbent assay for the organophosphorus insecticide bromophos-ethyl

Kwang Ok Kim, Yoo Jung Kim, Yong Tae Lee, Bruce D. Hammock, Hye Sung Lee

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative detection of the organophosphorus insecticide bromophos-ethyl. Four bromophos-ethyl derivatives (haptens) were synthesized and were coupled to carrier proteins through the pesticide thiophosphate group to use as an immunogen or as a coating antigen. Rabbits were immunized with either one of two haptens coupled to bovine serum albumin for production of polyclonal antibodies, and the sera were screened against one of the haptens coupled to ovalbumin. Using the serum with highest titer, an antigen-coated ELISA was developed, which showed an IC50 of 3.9 ng/mL with a detection limit of 0.3 ng/mL (20% inhibition). An antibody-coated ELISA using an enzyme tracer was also developed, which showed an IC50 of 6.5 ng/mL with a detection limit of 1.0 ng/mL (20% inhibition). The antibodies showed negligible cross-reactivity with other organophosphorus pesticides except with the insecticides bromophos-methyl and chlorpyrifos in the antibody-coated assay only. Recoveries of bromophosethyl from fortified crop and water samples ranged from 82 to 128% and from 95 to 127%, respectively.

Original languageEnglish (US)
Pages (from-to)6675-6682
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume50
Issue number23
DOIs
StatePublished - Nov 6 2002

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Keywords

  • Bromophos-ethyl
  • ELISA
  • Immunoassay
  • Insecticide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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