Development of an enzyme-linked immunosorbent assay for the organophosphorus insecticide bromophos

Won Chul Park, Young Ae Cho, Yoo Jung Kim, Bruce D. Hammock, Yong Tae Lee, Hye Sung Lee

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative detection of the organophosphorus insecticide bromophos. Three bromophos analogues (haptens) were synthesized and were coupled to carrier proteins to use as immunogens or coating antigens. Rabbits were immunized with either one of two haptens coupled to bovine serum albumin (BSA) for production of polyclonal antibodies, and the sera were screened against one of the haptens coupled to ovalbumin (OVA). Using the serum with highest specificity and an enzyme tracer, an antibody-coated ELISA was developed, which showed an IC50 of 40 ng/mL with a detection limit of 7 ng/mL. The antibodies in this assay showed negligible cross-reactivity with other organophosphorus pesticides except with the insecticides chlorpyrifos and fenitrothion.

Original languageEnglish (US)
Pages (from-to)1399-1403
Number of pages5
JournalBulletin of the Korean Chemical Society
Volume23
Issue number10
StatePublished - Oct 20 2002

Keywords

  • Bromophos
  • ELISA
  • Immunoassay
  • Insecticide

ASJC Scopus subject areas

  • Chemistry(all)

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    Park, W. C., Cho, Y. A., Kim, Y. J., Hammock, B. D., Lee, Y. T., & Lee, H. S. (2002). Development of an enzyme-linked immunosorbent assay for the organophosphorus insecticide bromophos. Bulletin of the Korean Chemical Society, 23(10), 1399-1403.