Development of an enzyme-linked immunosorbent assay for the detection of the triazole fungicide myclobutanil

András Székács, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) system for quantitative detection of myclobutanil was developed. Polyclonal antibodies were raised in rabbits against the haptenic compound 2-(4-chlorophenyl)-2-[(1,2,4-triazol-1-yl)methyl]hexanoic acid, conjugated to conalbumin through the carboxylic acid moiety. The standard curve based on a homologous system using the above hapten conjugated to bovine serum albumin displayed a detection range from 0.3 to 200 μg/ mL for myclobutanil with an IC50 value of 4.9 ± 0.5 μg/mL. The effects of various assay conditions, including pH, organic solvent content, sample matrix effects (surface water, soil and fruit extracts), and protein stabilizer, on the sensitivity have been evaluated. Cross-reactivities with various related fungicides, insecticides, pharmaceuticals, and intermediates containing triazole, imidazole, or pyrimidine groups were tested and found to be below 15%. The detection system appears to be a versatile device for the environmental monitoring of myclobutanil.

Original languageEnglish (US)
Pages (from-to)2083-2091
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume43
Issue number8
StatePublished - 1995

Keywords

  • Analytical chemistry
  • Biotechnology-based methods
  • ELISA
  • Fungicide
  • Immunoassay
  • Monitoring
  • Myclobutanil

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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