Abstract
A competitive enzyme-linked immunosorbent assay (ELISA) system for quantitative detection of myclobutanil was developed. Polyclonal antibodies were raised in rabbits against the haptenic compound 2-(4-chlorophenyl)-2-[(1,2,4-triazol-1-yl)methyl]hexanoic acid, conjugated to conalbumin through the carboxylic acid moiety. The standard curve based on a homologous system using the above hapten conjugated to bovine serum albumin displayed a detection range from 0.3 to 200 μg/ mL for myclobutanil with an IC50 value of 4.9 ± 0.5 μg/mL. The effects of various assay conditions, including pH, organic solvent content, sample matrix effects (surface water, soil and fruit extracts), and protein stabilizer, on the sensitivity have been evaluated. Cross-reactivities with various related fungicides, insecticides, pharmaceuticals, and intermediates containing triazole, imidazole, or pyrimidine groups were tested and found to be below 15%. The detection system appears to be a versatile device for the environmental monitoring of myclobutanil.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2083-2091 |
| Number of pages | 9 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 43 |
| Issue number | 8 |
| State | Published - 1995 |
Keywords
- Analytical chemistry
- Biotechnology-based methods
- ELISA
- Fungicide
- Immunoassay
- Monitoring
- Myclobutanil
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)