Development of an enzyme-linked immunosorbent assay for caprine arthritis-encephalitis virus

D. Archambault, Nancy East, K. Perk, J. E. Dahlberg

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Because relatively few caprine arthritis-encephalitis virus (CAEV)-infected animals exhibit clinical signs of illness, efforts to control and eradicate this virus will depend heavily on a sensitive diagnostic test that can be easily carried out. The currently utilized tests are of limited usefulness because of relatively low sensitivity or because of incomplete cross-reactivity of goat sera with heterologous test antigens. An enzyme-linked immunosorbent assay (ELISA) with purified CAEV antigen and biotin-avidin amplification steps was therefore developed and compared with a radioimmunoassay (RIA) against CAEV p28. Of over 500 sera tested, there was 99% concordance between the two tests. On the other hand, 23 of 24 sera obtained from animals with clinical signs of disease that were negative by agar gel immunodiffusion test (with ovine progressive pneumonia virus antigen) were positive by ELISA and RIA. These results suggest that an ELISA with CAEV antigen is superior to the agar gel immunodiffusion test and is easier and faster than an RIA, and therefore may be the method of choice for diagnosing CAEV infection.

Original languageEnglish (US)
Pages (from-to)971-975
Number of pages5
JournalJournal of Clinical Microbiology
Volume26
Issue number5
StatePublished - Jan 1 1988
Externally publishedYes

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Caprine Arthritis-Encephalitis Viruses
Enzyme-Linked Immunosorbent Assay
Radioimmunoassay
Immunodiffusion
Antigens
Agar
Gels
Serum
Heterophile Antigens
Viruses
Avidin
Virus Diseases
Biotin
Routine Diagnostic Tests
Goats
Sheep
Pneumonia

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Development of an enzyme-linked immunosorbent assay for caprine arthritis-encephalitis virus. / Archambault, D.; East, Nancy; Perk, K.; Dahlberg, J. E.

In: Journal of Clinical Microbiology, Vol. 26, No. 5, 01.01.1988, p. 971-975.

Research output: Contribution to journalArticle

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