Development of an Enzyme Immunoassay for Alternaria alternata f.sp. lycopersici Toxins

Ferenc Szurdoki, Eugene Trousdale, Shirley J. Gee, Barney Ward, Bruce D. Hammock, David G. Gilchrist

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

AAL toxins and fumonisins are sphinganine analog mycotoxins secreted by the fungal pathogens Alternaria alternata f.sp. lycopersici and Fusarium moniliforme, respectively. Consumption of fumonisin-contaminated maize has been reported to cause several animal diseases and has been correlated with high incidence of human esophageal cancer. Recent studies also demonstrate that both fumonisins and AAL compounds are phytotoxic to tomato, cytotoxic to cultured mammalian cells, and inhibit ceramide synthase in animals. Preliminary evidence indicates that AAL toxins are present in field-grown tomatoes, destined for processing, which show signs of the blackmold disease. An enzyme-linked immunosorbent assay (ELISA) would provide a rapid and inexpensive analytical tool for screening large numbers of food samples for AAL toxins. We report here the development of a class-selective ELISA for detection of AAL toxins at ppb to low ppm levels. Mice were inoculated with various protein conjugates of AAL compound TA and the elicited polyclonal antibodies were used for assay development. No cross-reactivities were found with sphinganine, the structurally related sphingoid base, or with fumonisin B1. The sensitivity and selectivity of the assay produced in this preliminary study indicate a high potential for development of ELISAs for the determination of AAL toxins in food and feed samples.

Original languageEnglish (US)
Pages (from-to)330-340
Number of pages11
JournalACS Symposium Series
Volume621
StatePublished - 1996

ASJC Scopus subject areas

  • Chemistry(all)

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