Development of an ELISA for the organophosphorus insecticide chlorpyrifos

Young Ae Cho, Hye Sung Lee, Eun Young Park, Yong Tae Lee, Bruce D. Hammock, Ki Chang Ahn, Jae Koo Lee

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

A selective enzyme-linked immunosorbent assay (ELISA) for the insecticide chlorpyrifos was developed. Four haptens for chlorpyrifos were synthesized and two of them were used as immunogens after coupling to keyhole limpet hemocyanin by two different approaches. Rabbits were immunized with either of them and the sera were screened against 4 haptens coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigen-coated ELISA was developed, which shows an I50 of 160 ppb with a detection limit of 10 ppb. An antibody-coated ELISA was also developed, which shows an I50 of 20 ppb with a detection limit of 0.1 ppb. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides except for insecticides chlorpyrifos-methyl and bromophos-ethyl, which makes these assays suitable for the selective detection of chlorpyrifos.

Original languageEnglish (US)
Pages (from-to)481-487
Number of pages7
JournalBulletin of the Korean Chemical Society
Volume23
Issue number3
StatePublished - Mar 20 2002

Keywords

  • Chlorpyrifos
  • ELISA
  • Immunoassay
  • Insecticide
  • Organophosphorus

ASJC Scopus subject areas

  • Chemistry(all)

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