Development of an ELISA for the organophosphorus insecticide chlorpyrifos

Young Ae Cho, Hye Sung Lee, Eun Young Park, Yong Tae Lee, Bruce D. Hammock, Ki Chang Ahn, Jae Koo Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A selective enzyme-linked immunosorbent assay (ELISA) for the insecticide chlorpyrifos was developed. Four haptens for chlorpyrifos were synthesized and two of them were used as immunogens after coupling to keyhole limpet hemocyanin by two different approaches. Rabbits were immunized with either of them and the sera were screened against 4 haptens coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigen-coated ELISA was developed, which shows an I50 of 160 ppb with a detection limit of 10 ppb. An antibody-coated ELISA was also developed, which shows an I50 of 20 ppb with a detection limit of 0.1 ppb. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides except for insecticides chlorpyrifos-methyl and bromophos-ethyl, which makes these assays suitable for the selective detection of chlorpyrifos.

Original languageEnglish (US)
Pages (from-to)481-487
Number of pages7
JournalBulletin of the Korean Chemical Society
Volume23
Issue number3
StatePublished - Mar 20 2002

Fingerprint

Chlorpyrifos
Immunosorbents
Insecticides
Assays
Haptens
Enzymes
Antibodies
Ovalbumin
Pesticides
Antigens

Keywords

  • Chlorpyrifos
  • ELISA
  • Immunoassay
  • Insecticide
  • Organophosphorus

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Cho, Y. A., Lee, H. S., Park, E. Y., Lee, Y. T., Hammock, B. D., Ahn, K. C., & Lee, J. K. (2002). Development of an ELISA for the organophosphorus insecticide chlorpyrifos. Bulletin of the Korean Chemical Society, 23(3), 481-487.

Development of an ELISA for the organophosphorus insecticide chlorpyrifos. / Cho, Young Ae; Lee, Hye Sung; Park, Eun Young; Lee, Yong Tae; Hammock, Bruce D.; Ahn, Ki Chang; Lee, Jae Koo.

In: Bulletin of the Korean Chemical Society, Vol. 23, No. 3, 20.03.2002, p. 481-487.

Research output: Contribution to journalArticle

Cho, YA, Lee, HS, Park, EY, Lee, YT, Hammock, BD, Ahn, KC & Lee, JK 2002, 'Development of an ELISA for the organophosphorus insecticide chlorpyrifos', Bulletin of the Korean Chemical Society, vol. 23, no. 3, pp. 481-487.
Cho YA, Lee HS, Park EY, Lee YT, Hammock BD, Ahn KC et al. Development of an ELISA for the organophosphorus insecticide chlorpyrifos. Bulletin of the Korean Chemical Society. 2002 Mar 20;23(3):481-487.
Cho, Young Ae ; Lee, Hye Sung ; Park, Eun Young ; Lee, Yong Tae ; Hammock, Bruce D. ; Ahn, Ki Chang ; Lee, Jae Koo. / Development of an ELISA for the organophosphorus insecticide chlorpyrifos. In: Bulletin of the Korean Chemical Society. 2002 ; Vol. 23, No. 3. pp. 481-487.
@article{423669f5c0c044ac964ad7ca3d8606fd,
title = "Development of an ELISA for the organophosphorus insecticide chlorpyrifos",
abstract = "A selective enzyme-linked immunosorbent assay (ELISA) for the insecticide chlorpyrifos was developed. Four haptens for chlorpyrifos were synthesized and two of them were used as immunogens after coupling to keyhole limpet hemocyanin by two different approaches. Rabbits were immunized with either of them and the sera were screened against 4 haptens coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigen-coated ELISA was developed, which shows an I50 of 160 ppb with a detection limit of 10 ppb. An antibody-coated ELISA was also developed, which shows an I50 of 20 ppb with a detection limit of 0.1 ppb. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides except for insecticides chlorpyrifos-methyl and bromophos-ethyl, which makes these assays suitable for the selective detection of chlorpyrifos.",
keywords = "Chlorpyrifos, ELISA, Immunoassay, Insecticide, Organophosphorus",
author = "Cho, {Young Ae} and Lee, {Hye Sung} and Park, {Eun Young} and Lee, {Yong Tae} and Hammock, {Bruce D.} and Ahn, {Ki Chang} and Lee, {Jae Koo}",
year = "2002",
month = "3",
day = "20",
language = "English (US)",
volume = "23",
pages = "481--487",
journal = "Bulletin of the Korean Chemical Society",
issn = "0253-2964",
publisher = "Korean Chemical Society",
number = "3",

}

TY - JOUR

T1 - Development of an ELISA for the organophosphorus insecticide chlorpyrifos

AU - Cho, Young Ae

AU - Lee, Hye Sung

AU - Park, Eun Young

AU - Lee, Yong Tae

AU - Hammock, Bruce D.

AU - Ahn, Ki Chang

AU - Lee, Jae Koo

PY - 2002/3/20

Y1 - 2002/3/20

N2 - A selective enzyme-linked immunosorbent assay (ELISA) for the insecticide chlorpyrifos was developed. Four haptens for chlorpyrifos were synthesized and two of them were used as immunogens after coupling to keyhole limpet hemocyanin by two different approaches. Rabbits were immunized with either of them and the sera were screened against 4 haptens coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigen-coated ELISA was developed, which shows an I50 of 160 ppb with a detection limit of 10 ppb. An antibody-coated ELISA was also developed, which shows an I50 of 20 ppb with a detection limit of 0.1 ppb. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides except for insecticides chlorpyrifos-methyl and bromophos-ethyl, which makes these assays suitable for the selective detection of chlorpyrifos.

AB - A selective enzyme-linked immunosorbent assay (ELISA) for the insecticide chlorpyrifos was developed. Four haptens for chlorpyrifos were synthesized and two of them were used as immunogens after coupling to keyhole limpet hemocyanin by two different approaches. Rabbits were immunized with either of them and the sera were screened against 4 haptens coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigen-coated ELISA was developed, which shows an I50 of 160 ppb with a detection limit of 10 ppb. An antibody-coated ELISA was also developed, which shows an I50 of 20 ppb with a detection limit of 0.1 ppb. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides except for insecticides chlorpyrifos-methyl and bromophos-ethyl, which makes these assays suitable for the selective detection of chlorpyrifos.

KW - Chlorpyrifos

KW - ELISA

KW - Immunoassay

KW - Insecticide

KW - Organophosphorus

UR - http://www.scopus.com/inward/record.url?scp=0037139884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037139884&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0037139884

VL - 23

SP - 481

EP - 487

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

SN - 0253-2964

IS - 3

ER -