Development of an ELISA for the detection of the residues of the insecticide imidacloprid in agricultural and environmental samples

Jae Koo Lee, Ki Chang Ahn, Oee Suk Park, Shin Young Kang, Bruce D. Hammock

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) for the chloronicotinyl insecticide imidacloprid was developed using a polyclonal antibody produced against a hapten conjugated through the imidazolidine to keyhole limpet hemocyanin. In the standard curve of imidacloprid, an IC50 of 17.3 ng/mL was obtained using a competitive heterologous system at pH 10. Very low cross-reactivity was found for some structurally related compounds including the insecticide thiacloprid. The high cross-reactivity with a metabolite containing the carbonyl group in the imidazolidine moiety suggests the involvement of its polarity and stereochemical fitness in forming the antibody-antigen complex. The effects of various assay conditions, including organic solvents, detergent content, salt concentration, and pH on the sensitivity were evaluated. High-performance liquid chromatography was run for comparison to validate the ELISA with fortified water samples, the correlation being 0.997 - 0.998 (n = 15) with a slope of 1.10 - 1.38. The ELISA turned out to be a convenient tool for monitoring imidacloprid residues in agricultural and environmental samples.

Original languageEnglish (US)
Pages (from-to)2159-2167
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume49
Issue number5
DOIs
StatePublished - 2001

Keywords

  • Agricultural/environmental
  • Cross-reactivity
  • ELISA
  • Imidacloprid
  • Monitoring
  • Polyclonal

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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