Development of a time-gated system for Raman spectroscopy of biological samples

Florian Knorr, Zachary J. Smith, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.

Original languageEnglish (US)
Pages (from-to)20049-20058
Number of pages10
JournalOptics Express
Volume18
Issue number19
DOIs
StatePublished - Sep 13 2010

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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