Abstract
A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 20049-20058 |
| Number of pages | 10 |
| Journal | Optics Express |
| Volume | 18 |
| Issue number | 19 |
| DOIs | |
| State | Published - Sep 13 2010 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Development of a time-gated system for Raman spectroscopy of biological samples. / Knorr, Florian; Smith, Zachary J.; Wachsmann-Hogiu, Sebastian.
In: Optics Express, Vol. 18, No. 19, 13.09.2010, p. 20049-20058.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of a time-gated system for Raman spectroscopy of biological samples
AU - Knorr, Florian
AU - Smith, Zachary J.
AU - Wachsmann-Hogiu, Sebastian
PY - 2010/9/13
Y1 - 2010/9/13
N2 - A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.
AB - A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.
UR - http://www.scopus.com/inward/record.url?scp=77957001485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957001485&partnerID=8YFLogxK
U2 - 10.1364/OE.18.020049
DO - 10.1364/OE.18.020049
M3 - Article
C2 - 20940895
AN - SCOPUS:77957001485
VL - 18
SP - 20049
EP - 20058
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 19
ER -