Development of a time-gated system for Raman spectroscopy of biological samples

Florian Knorr, Zachary J. Smith, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.

Original languageEnglish (US)
Pages (from-to)20049-20058
Number of pages10
JournalOptics Express
Volume18
Issue number19
DOIs
StatePublished - Sep 13 2010

Fingerprint

Raman spectroscopy
optical switching
stems
toluene
conservation
recording
Raman spectra
fluorescence
thresholds
geometry
pulses
energy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Development of a time-gated system for Raman spectroscopy of biological samples. / Knorr, Florian; Smith, Zachary J.; Wachsmann-Hogiu, Sebastian.

In: Optics Express, Vol. 18, No. 19, 13.09.2010, p. 20049-20058.

Research output: Contribution to journalArticle

Knorr, Florian ; Smith, Zachary J. ; Wachsmann-Hogiu, Sebastian. / Development of a time-gated system for Raman spectroscopy of biological samples. In: Optics Express. 2010 ; Vol. 18, No. 19. pp. 20049-20058.
@article{10c089e8a481410a9056e66c6aa76afd,
title = "Development of a time-gated system for Raman spectroscopy of biological samples",
abstract = "A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5{\%} peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.",
author = "Florian Knorr and Smith, {Zachary J.} and Sebastian Wachsmann-Hogiu",
year = "2010",
month = "9",
day = "13",
doi = "10.1364/OE.18.020049",
language = "English (US)",
volume = "18",
pages = "20049--20058",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "19",

}

TY - JOUR

T1 - Development of a time-gated system for Raman spectroscopy of biological samples

AU - Knorr, Florian

AU - Smith, Zachary J.

AU - Wachsmann-Hogiu, Sebastian

PY - 2010/9/13

Y1 - 2010/9/13

N2 - A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.

AB - A time gating system has been constructed that is capable of recording high quality Raman spectra of highly fluorescing biological samples while operating below the photodamage threshold. Using a collinear gating geometry and careful attention to power conservation, we have achieved all-optical switching with a one picosecond gating time and 5% peak gating efficiency. The energy per pulse in this instrument is more than 3 orders of magnitude weaker than previous reports. Using this system we have performed proof-of-concept experiments on a sample composed of perylene dissolved in toluene, and the stem of a Jasminum multiflorum plant, the latter case being particularly important for the study of plants used in production of cellulosic biofuels. In both cases, a high SNR spectrum of the high-wavenumber region of the spectrum was recorded in the presence of an overwhelming fluorescence background.

UR - http://www.scopus.com/inward/record.url?scp=77957001485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957001485&partnerID=8YFLogxK

U2 - 10.1364/OE.18.020049

DO - 10.1364/OE.18.020049

M3 - Article

C2 - 20940895

AN - SCOPUS:77957001485

VL - 18

SP - 20049

EP - 20058

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 19

ER -