Development of a sequence typing scheme for differentiation of Salmonella Enteritidis strains

Bertrand Tankouo-Sandjong, Hailu Kinde, Isha Wallace

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A DNA sequence typing scheme based on the caiC and SEN0629 loci was developed for differentiation of Salmonella Enteritidis strains and validated using a diverse collection of 102 isolates representing 38 phage types from different sources, year of isolation, geographical locations and epidemiological backgrounds. caiC encodes a probable crotonobetaine/carnitine-CoA ligase, and SEN0629 is a pseudogene. Our system allowed for discrimination of 16 sequence types (STs) among the 102 isolates analysed and intraphage type differentiation. Our findings also suggested that the stability of phage typing may be adversely affected by the occurrence of phage type conversion events. During a confirmatory phage typing analysis performed by a reference laboratory, 13 of 31 S. Enteritidis strains representing nine phage types were assigned phage types that differed from the ones originally determined by the same reference laboratory. It is possible that this phenomenon passes largely unrecognized in reference laboratories performing routine phage typing analyses. Our results demonstrate that phage typing is an unstable system displaying limited reproducibility and that the two-loci sequence typing scheme is highly discriminatory, stable, truly portable and has the potential to become the new gold standard for epidemiological typing of S. Enteritidis strains.

Original languageEnglish (US)
Pages (from-to)165-175
Number of pages11
JournalFEMS Microbiology Letters
Volume331
Issue number2
DOIs
StatePublished - Jun 2012

Fingerprint

Bacteriophage Typing
Salmonella enteritidis
Bacteriophages
Pseudogenes
DNA Fingerprinting
Carnitine
Coenzyme A
Ligases

Keywords

  • caiC
  • Phage typing
  • Salmonella Enteritidis
  • SEN0629
  • Sequence typing

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

Development of a sequence typing scheme for differentiation of Salmonella Enteritidis strains. / Tankouo-Sandjong, Bertrand; Kinde, Hailu; Wallace, Isha.

In: FEMS Microbiology Letters, Vol. 331, No. 2, 06.2012, p. 165-175.

Research output: Contribution to journalArticle

Tankouo-Sandjong, Bertrand ; Kinde, Hailu ; Wallace, Isha. / Development of a sequence typing scheme for differentiation of Salmonella Enteritidis strains. In: FEMS Microbiology Letters. 2012 ; Vol. 331, No. 2. pp. 165-175.
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