Development of a semiautomated microassay for bovine immunoglobulin E

definition and standardization.

Laurel J Gershwin, B. S. Dygert

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Although the serum immunoglobulin (Ig) E concentration is a critical and valuable measurement in parasitic infections and allergic diseases, little has been reported on it in cattle. We have developed an enzyme-linked immunosorbent assay to measure serum concentrations of bovine IgE. This assay was developed and standardized, using the statistical guidelines of the Bureau of Biological Standardization of National Institutes of Health. A reference serum used to standardize results was assigned a value of 100 IgE U/ml. Absorbance values for test samples were converted into IgE units by comparison with a standard curve, generated by dilutions of this reference serum. Serum samples from 160 adult cows and calves had a geometric mean of 17.8 IgE U/ml. The IgE values were higher in calves from parasite-infested pastures than in parasite-free calves and adult cows. This assay is the first procedure appropriate for multiautomated analysis of IgE in cattle.

Original languageEnglish (US)
Pages (from-to)891-895
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume44
Issue number5
StatePublished - May 1983

Fingerprint

immunoglobulin E
standardization
Immunoglobulin E
calves
cattle
National Institutes of Health
parasites
cows
Serum
assays
parasitoses
absorbance
pastures
enzyme-linked immunosorbent assay
Parasites
sampling
Parasitic Diseases
National Institutes of Health (U.S.)
Cerebral Palsy
testing

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Development of a semiautomated microassay for bovine immunoglobulin E : definition and standardization. / Gershwin, Laurel J; Dygert, B. S.

In: American Journal of Veterinary Research, Vol. 44, No. 5, 05.1983, p. 891-895.

Research output: Contribution to journalArticle

@article{05de5c1d0fdb4aa49e3f6adef54b1ef7,
title = "Development of a semiautomated microassay for bovine immunoglobulin E: definition and standardization.",
abstract = "Although the serum immunoglobulin (Ig) E concentration is a critical and valuable measurement in parasitic infections and allergic diseases, little has been reported on it in cattle. We have developed an enzyme-linked immunosorbent assay to measure serum concentrations of bovine IgE. This assay was developed and standardized, using the statistical guidelines of the Bureau of Biological Standardization of National Institutes of Health. A reference serum used to standardize results was assigned a value of 100 IgE U/ml. Absorbance values for test samples were converted into IgE units by comparison with a standard curve, generated by dilutions of this reference serum. Serum samples from 160 adult cows and calves had a geometric mean of 17.8 IgE U/ml. The IgE values were higher in calves from parasite-infested pastures than in parasite-free calves and adult cows. This assay is the first procedure appropriate for multiautomated analysis of IgE in cattle.",
author = "Gershwin, {Laurel J} and Dygert, {B. S.}",
year = "1983",
month = "5",
language = "English (US)",
volume = "44",
pages = "891--895",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "5",

}

TY - JOUR

T1 - Development of a semiautomated microassay for bovine immunoglobulin E

T2 - definition and standardization.

AU - Gershwin, Laurel J

AU - Dygert, B. S.

PY - 1983/5

Y1 - 1983/5

N2 - Although the serum immunoglobulin (Ig) E concentration is a critical and valuable measurement in parasitic infections and allergic diseases, little has been reported on it in cattle. We have developed an enzyme-linked immunosorbent assay to measure serum concentrations of bovine IgE. This assay was developed and standardized, using the statistical guidelines of the Bureau of Biological Standardization of National Institutes of Health. A reference serum used to standardize results was assigned a value of 100 IgE U/ml. Absorbance values for test samples were converted into IgE units by comparison with a standard curve, generated by dilutions of this reference serum. Serum samples from 160 adult cows and calves had a geometric mean of 17.8 IgE U/ml. The IgE values were higher in calves from parasite-infested pastures than in parasite-free calves and adult cows. This assay is the first procedure appropriate for multiautomated analysis of IgE in cattle.

AB - Although the serum immunoglobulin (Ig) E concentration is a critical and valuable measurement in parasitic infections and allergic diseases, little has been reported on it in cattle. We have developed an enzyme-linked immunosorbent assay to measure serum concentrations of bovine IgE. This assay was developed and standardized, using the statistical guidelines of the Bureau of Biological Standardization of National Institutes of Health. A reference serum used to standardize results was assigned a value of 100 IgE U/ml. Absorbance values for test samples were converted into IgE units by comparison with a standard curve, generated by dilutions of this reference serum. Serum samples from 160 adult cows and calves had a geometric mean of 17.8 IgE U/ml. The IgE values were higher in calves from parasite-infested pastures than in parasite-free calves and adult cows. This assay is the first procedure appropriate for multiautomated analysis of IgE in cattle.

UR - http://www.scopus.com/inward/record.url?scp=0020757069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020757069&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 891

EP - 895

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 5

ER -