Development of a selective enzyme-linked immunosorbent assay for 1-naphthol - The major metabolite of carbaryl (1-naphthyl N-methylcarbamate)

Petra M. Krämer, Maria Pilar Marco, Bruce D. Hammock

Research output: Contribution to journalArticle

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Abstract

A selective enzyme-linked immunosorbent assay for 1-naphthol was developed. Eight rabbits were immunized with either [(5-hydroxy-2-naphthylenyl)oxy]acetic acid (2b)-BSA, [(5-hydroxy-1-naphthylenyl)oxy]acetic acid (4)-BSA, or 5-hydroxy-2-naphthoic acid (7)-KLH, and all sera were screened against 14 different coating antigens. From this screening procedure, five assays could be developed, using two 7-KLH antisera, 3907 and 3909. The assay with the least interference and the best reliability (7-KLH antiserum 3907, 1:9000, and coating antigen 2b-BSA, 1.5 μg/mL) was further optimized and characterized. It showed an I50 value of about 72 μg/L, with a range of detection from 10 to 1000 μg/L. There was no cross-reactivity with carbaryl, which makes this assay very suitable for the selective detection of 1-naphthol, for example, in undiluted human urine and in soil extract. The assay was tested in organic solvents and showed a tolerance of at least 10% solvent (acetone, acetonitrile, methanol).

Original languageEnglish (US)
Pages (from-to)934-943
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume42
Issue number4
StatePublished - 1994

Fingerprint

1-naphthol
Carbaryl
methylcarbamates
Immunosorbents
carbaryl
Metabolites
Acetic Acid
Immune Sera
Assays
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
metabolites
Antigens
assays
Enzymes
coatings
acetic acid
Methanol
antiserum
Soil

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Development of a selective enzyme-linked immunosorbent assay for 1-naphthol - The major metabolite of carbaryl (1-naphthyl N-methylcarbamate). / Krämer, Petra M.; Marco, Maria Pilar; Hammock, Bruce D.

In: Journal of Agricultural and Food Chemistry, Vol. 42, No. 4, 1994, p. 934-943.

Research output: Contribution to journalArticle

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