Abstract
A selective enzyme-linked immunosorbent assay for 1-naphthol was developed. Eight rabbits were immunized with either [(5-hydroxy-2-naphthylenyl)oxy]acetic acid (2b)-BSA, [(5-hydroxy-1-naphthylenyl)oxy]acetic acid (4)-BSA, or 5-hydroxy-2-naphthoic acid (7)-KLH, and all sera were screened against 14 different coating antigens. From this screening procedure, five assays could be developed, using two 7-KLH antisera, 3907 and 3909. The assay with the least interference and the best reliability (7-KLH antiserum 3907, 1:9000, and coating antigen 2b-BSA, 1.5 μg/mL) was further optimized and characterized. It showed an I50 value of about 72 μg/L, with a range of detection from 10 to 1000 μg/L. There was no cross-reactivity with carbaryl, which makes this assay very suitable for the selective detection of 1-naphthol, for example, in undiluted human urine and in soil extract. The assay was tested in organic solvents and showed a tolerance of at least 10% solvent (acetone, acetonitrile, methanol).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 934-943 |
| Number of pages | 10 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 42 |
| Issue number | 4 |
| State | Published - 1994 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
Development of a selective enzyme-linked immunosorbent assay for 1-naphthol - The major metabolite of carbaryl (1-naphthyl N-methylcarbamate). / Krämer, Petra M.; Marco, Maria Pilar; Hammock, Bruce D.
In: Journal of Agricultural and Food Chemistry, Vol. 42, No. 4, 1994, p. 934-943.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a selective enzyme-linked immunosorbent assay for 1-naphthol - The major metabolite of carbaryl (1-naphthyl N-methylcarbamate)
AU - Krämer, Petra M.
AU - Marco, Maria Pilar
AU - Hammock, Bruce D.
PY - 1994
Y1 - 1994
N2 - A selective enzyme-linked immunosorbent assay for 1-naphthol was developed. Eight rabbits were immunized with either [(5-hydroxy-2-naphthylenyl)oxy]acetic acid (2b)-BSA, [(5-hydroxy-1-naphthylenyl)oxy]acetic acid (4)-BSA, or 5-hydroxy-2-naphthoic acid (7)-KLH, and all sera were screened against 14 different coating antigens. From this screening procedure, five assays could be developed, using two 7-KLH antisera, 3907 and 3909. The assay with the least interference and the best reliability (7-KLH antiserum 3907, 1:9000, and coating antigen 2b-BSA, 1.5 μg/mL) was further optimized and characterized. It showed an I50 value of about 72 μg/L, with a range of detection from 10 to 1000 μg/L. There was no cross-reactivity with carbaryl, which makes this assay very suitable for the selective detection of 1-naphthol, for example, in undiluted human urine and in soil extract. The assay was tested in organic solvents and showed a tolerance of at least 10% solvent (acetone, acetonitrile, methanol).
AB - A selective enzyme-linked immunosorbent assay for 1-naphthol was developed. Eight rabbits were immunized with either [(5-hydroxy-2-naphthylenyl)oxy]acetic acid (2b)-BSA, [(5-hydroxy-1-naphthylenyl)oxy]acetic acid (4)-BSA, or 5-hydroxy-2-naphthoic acid (7)-KLH, and all sera were screened against 14 different coating antigens. From this screening procedure, five assays could be developed, using two 7-KLH antisera, 3907 and 3909. The assay with the least interference and the best reliability (7-KLH antiserum 3907, 1:9000, and coating antigen 2b-BSA, 1.5 μg/mL) was further optimized and characterized. It showed an I50 value of about 72 μg/L, with a range of detection from 10 to 1000 μg/L. There was no cross-reactivity with carbaryl, which makes this assay very suitable for the selective detection of 1-naphthol, for example, in undiluted human urine and in soil extract. The assay was tested in organic solvents and showed a tolerance of at least 10% solvent (acetone, acetonitrile, methanol).
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UR - http://www.scopus.com/inward/citedby.url?scp=0000641538&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0000641538
VL - 42
SP - 934
EP - 943
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 4
ER -