Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp

Pedro H.O. Viadanna, Tim Miller-Morgan, Trace Peterson, Keith Way, David M. Stone, Gary D. Marty, Fabiana Pilarski, Ronald Hedrick, Thomas B. Waltzek

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cyprinid herpesvirus 1 (CyHV1) infects all scaled and color varieties of common carp Cyprinus carpio, including koi. While it is most often associated with unsightly growths known as 'carp pox,' the underlying lesion (epidermal hyperplasia) can arise from a variety of disease processes. CyHV1-induced epidermal hyperplasia may occur transiently in response to water temperature, and thus histopathology cannot be used in isolation to assess CyHV1 infection status. To address this problem, here we describe a PCR assay targeted to the putative thymidine kinase gene of CyHV1. The PCR assay generates a 141 bp amplicon and reliably detects down to 10 copies of control plasmid DNA sequence (analytic sensitivity). The PCR does not cross-detect genomic DNA from cyprinid herpesvirus 2 and 3 (analytic specificity). The CyHV1 PCR effectively detected viral DNA in koi and common carp sampled from various locations in the UK, USA, Brazil, and Japan. Viral DNA was detected in both normal appearing and grossly affected epidermal tissues from koi experiencing natural epizootics. The new CyHV1 PCR provides an additional approach to histopathology for the rapid detection of CyHV1. Analysis of the thymidine kinase gene sequences determined for 7 PCR-positive carp originating from disparate geographical regions identified 3 sequence types, with 1 type occurring in both koi and common carp.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalDiseases of Aquatic Organisms
Volume123
Issue number1
DOIs
StatePublished - Feb 8 2017

Fingerprint

Cyprinid herpesvirus 1
koi
cyprinid
Cyprinus carpio
assay
assays
thymidine kinase
DNA
histopathology
hyperplasia
carp
Cyprinid herpesvirus 2
Cyprinid herpesvirus 3
colored varieties
nucleotide sequences
gene
geographical region
lesions (animal)
plasmid
lesion

Keywords

  • Cyprinus carpio
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Viadanna, P. H. O., Miller-Morgan, T., Peterson, T., Way, K., Stone, D. M., Marty, G. D., ... Waltzek, T. B. (2017). Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp. Diseases of Aquatic Organisms, 123(1), 19-27. https://doi.org/10.3354/dao03066

Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp. / Viadanna, Pedro H.O.; Miller-Morgan, Tim; Peterson, Trace; Way, Keith; Stone, David M.; Marty, Gary D.; Pilarski, Fabiana; Hedrick, Ronald; Waltzek, Thomas B.

In: Diseases of Aquatic Organisms, Vol. 123, No. 1, 08.02.2017, p. 19-27.

Research output: Contribution to journalArticle

Viadanna, PHO, Miller-Morgan, T, Peterson, T, Way, K, Stone, DM, Marty, GD, Pilarski, F, Hedrick, R & Waltzek, TB 2017, 'Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp', Diseases of Aquatic Organisms, vol. 123, no. 1, pp. 19-27. https://doi.org/10.3354/dao03066
Viadanna PHO, Miller-Morgan T, Peterson T, Way K, Stone DM, Marty GD et al. Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp. Diseases of Aquatic Organisms. 2017 Feb 8;123(1):19-27. https://doi.org/10.3354/dao03066
Viadanna, Pedro H.O. ; Miller-Morgan, Tim ; Peterson, Trace ; Way, Keith ; Stone, David M. ; Marty, Gary D. ; Pilarski, Fabiana ; Hedrick, Ronald ; Waltzek, Thomas B. / Development of a PCR assay to detect cyprinid herpesvirus 1 in koi and common carp. In: Diseases of Aquatic Organisms. 2017 ; Vol. 123, No. 1. pp. 19-27.
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