Development of a multi-modal tissue diagnostic system high frequency ultrasound and photoacoustic with lifetime fluorescence spectroscopy

Yang Sun, Douglas N. Stephens, Jesung Park, Yinghua Sun, Laura Marcu, Jonathan M. Cannata, K. Kirk Shung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations


We report the development and validate a multi-modal tissue diagnostic technology, which combines three complementary techniques into one system including ultrasound backscatter microscopy (UBM), photoacoustic imaging (PAI), and time-resolved laser-induced fluorescence spectroscopy (TRLIFS). UBM enables the reconstruction of the tissue microanatomy. PAI maps the optical absorption heterogeneity of the tissue associated with structure information and has the potential to provide functional imaging of the tissue. Examination of the UBM and PAI images allows for localization of regions of interest for TR-LIFS evaluation of the tissue composition. The hybrid probe consists of a single element ring transducer with concentric fiber optics for multi-modal data acquisition. Validation and characterization of the multi-modal system and ultrasonic, photoacoustic, and spectroscopic data coregistration were conducted in a physical phantom with properties of ultrasound scattering, optical absorption, and fluorescence. The UBM system with the 41 MHz ring transducer can reach the axial and lateral resolution of 30 and 65 μm, respectively. The PAI system with 532 nm excitation light from a Nd:YAG laser shows great contrast for the distribution of optical absorbers. The TR-LIFS system records the fluorescence decay with the time resolution of ∼300 ps and a high sensitivity of nM concentration range. Biological phantom constructed with different types of tissues (tendon and fat) was used to demonstrate the complementary information provided by the three modalities. Fluorescence spectra and lifetimes were compared to differentiate chemical composition of tissues at the regions of interest determined by the coregistered high resolution UBM and PAI image. Current results demonstrate that the fusion of these techniques enables sequentially detection of functional, morphological, and compositional features of biological tissue, suggesting potential applications in diagnosis of tumors and atherosclerotic plaques.

Original languageEnglish (US)
Title of host publicationProceedings - IEEE Ultrasonics Symposium
Number of pages4
StatePublished - 2008
Event2008 IEEE International Ultrasonics Symposium, IUS 2008 - Beijing, China
Duration: Nov 2 2008Nov 5 2008


Other2008 IEEE International Ultrasonics Symposium, IUS 2008


  • Data coregistration
  • Multi-modal system
  • Photoacoustic imaging
  • Time-resolved fluorescence spcetroscopy
  • Ultrasound backscatter microscopy

ASJC Scopus subject areas

  • Acoustics and Ultrasonics


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