Development of a HPLC/tandem-MS method for the analysis of the larvicides methoprene, hydroprene, and kinoprene at trace levels using diels-alder derivatization

Pavel A. Aronov, Katja Dettmer, Julie A. Christiansen, Anthony J. Cornel, Bruce D. Hammock

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The invasion and subsequent spread of the mosquito-borne West Nile virus in the United States has resulted in increased use of methoprene. With the increased need for sensitive detection and monitoring of methoprene in the environment, an analytical LC/ESI-MS/MS method has been developed for the analysis of methoprene and two analogues, kinoprene and hydroprene, in water. To improve the ionization efficiency of the nonpolar analytes, a derivatization step with the Cookson-type reagent 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) was used. Derivatization improved the limit of detection 100-fold. For tandem MS analyses, limits of detection in environmental water samples (S/N = 3) are about 6 pg/mL for methoprene and 20 pg/mL for kinoprene and hydroprene, resulting in limits of quantification (S/N = 10) of 20 pg/mL for methoprene and 60 pg/mL for hydroprene and kinoprene extracted from 10 mL of water. This method was applied to measure methoprene concentrations in water samples from a treated site.

Original languageEnglish (US)
Pages (from-to)3306-3312
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number9
DOIs
StatePublished - May 4 2005

Fingerprint

hydroprene
Methoprene
larvicides
methoprene
derivatization
High Pressure Liquid Chromatography
Water
Limit of Detection
detection limit
water
methodology
West Nile virus
Culicidae
Viruses
ionization
Ionization
ethyl-3,7,11-trimethyl-2,4-dodecadienoate
sampling
Monitoring
monitoring

Keywords

  • Cookson-type reagent
  • ESI-MS
  • Hydroprene
  • Kinoprene
  • Methoprene
  • West Nile virus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Development of a HPLC/tandem-MS method for the analysis of the larvicides methoprene, hydroprene, and kinoprene at trace levels using diels-alder derivatization. / Aronov, Pavel A.; Dettmer, Katja; Christiansen, Julie A.; Cornel, Anthony J.; Hammock, Bruce D.

In: Journal of Agricultural and Food Chemistry, Vol. 53, No. 9, 04.05.2005, p. 3306-3312.

Research output: Contribution to journalArticle

Aronov, Pavel A. ; Dettmer, Katja ; Christiansen, Julie A. ; Cornel, Anthony J. ; Hammock, Bruce D. / Development of a HPLC/tandem-MS method for the analysis of the larvicides methoprene, hydroprene, and kinoprene at trace levels using diels-alder derivatization. In: Journal of Agricultural and Food Chemistry. 2005 ; Vol. 53, No. 9. pp. 3306-3312.
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