Development of a homogeneous phosphorescent immunoassay for the detection of polychlorinated dibenzo-p-dioxins

E. G. Matveeva, E. V. Gribkova, J. R. Sanborn, S. J. Gee, B. D. Hammock, A. P. Savitsky

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A homogeneous immunoassay for dioxins was developed using a phosphorescent label for detection. A dioxin derivative was conjugated to Pt-coproporphyrin. In the assay, when the antibodies against dioxin (DD3) were bound to the phosphorescent conjugate, the signal from Pt-coproporphyrin was quenched. The interaction between antibody and the conjugate was studied by time-resolved luminescence spectroscopy. As concentrations of dioxin standards increased from 39 pg/well to 2.5 ng/well the lifetime of the phosphorescence of the shortlived component increased from 25.6 microseconds to 58.9 microseconds. This increase in half-life was associated with a dose dependent quenching of the phosphorescence. The inhibition obtained is similar to that for a reported enzyme-based immunoassay, but the data were obtained in minutes instead of hours.

Original languageEnglish (US)
Pages (from-to)2311-2320
Number of pages10
JournalAnalytical Letters
Volume34
Issue number13
DOIs
StatePublished - 2001

Keywords

  • Homogeneous immunoassay
  • Phosphorescence
  • Polychlorinated dibenzo-p-dioxins
  • Pt-coproporphyrin
  • Time-resolved luminescence spectroscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Analytical Chemistry

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    Matveeva, E. G., Gribkova, E. V., Sanborn, J. R., Gee, S. J., Hammock, B. D., & Savitsky, A. P. (2001). Development of a homogeneous phosphorescent immunoassay for the detection of polychlorinated dibenzo-p-dioxins. Analytical Letters, 34(13), 2311-2320. https://doi.org/10.1081/AL-100107297