Abstract
A homogeneous immunoassay for dioxins was developed using a phosphorescent label for detection. A dioxin derivative was conjugated to Pt-coproporphyrin. In the assay, when the antibodies against dioxin (DD3) were bound to the phosphorescent conjugate, the signal from Pt-coproporphyrin was quenched. The interaction between antibody and the conjugate was studied by time-resolved luminescence spectroscopy. As concentrations of dioxin standards increased from 39 pg/well to 2.5 ng/well the lifetime of the phosphorescence of the shortlived component increased from 25.6 microseconds to 58.9 microseconds. This increase in half-life was associated with a dose dependent quenching of the phosphorescence. The inhibition obtained is similar to that for a reported enzyme-based immunoassay, but the data were obtained in minutes instead of hours.
Original language | English (US) |
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Pages (from-to) | 2311-2320 |
Number of pages | 10 |
Journal | Analytical Letters |
Volume | 34 |
Issue number | 13 |
DOIs | |
State | Published - 2001 |
Keywords
- Homogeneous immunoassay
- Phosphorescence
- Polychlorinated dibenzo-p-dioxins
- Pt-coproporphyrin
- Time-resolved luminescence spectroscopy
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Analytical Chemistry