Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide

Xiude Hua, Xiaofeng Liu, Haiyan Shi, Yanru Wang, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to detect organophosphorus pesticides using a phage-borne peptide that was isolated from a cyclic 8-residue peptide phage library. The IC50 values of the phage ELISA ranged from 1.4 to 92.1 μg L-1 for eight organophosphorus pesticides (parathion-methyl, parathion, fenitrothion, cyanophos, EPN, paraoxon-methyl, paraoxon, fenitrooxon). The sensitivity was improved 120- and 2-fold compared to conventional homologous and heterologous ELISA, respectively. The selectivity of the phage ELISA was evaluated by measuring its cross-reactivity with 23 organophosphorus pesticides, among which eight were the main cross-reactants. The spike recoveries were between 66.1% and 101.6% for the detection of single pesticide residues of parathion-methyl, parathion and fenitrothion in Chinese cabbage, apple and greengroceries, and all of the coefficients of variation were less than or equal to 15.9%. Moreover, the phage ELISA results were validated by gas chromatography. The results indicate that isolating phage-borne peptides from phage display libraries is an alternative method for the development of a heterologous immunoassay and that the developed assay has a lower limit of detection than the chemically synthesized competitor assay.

Original languageEnglish (US)
Pages (from-to)42445-42453
Number of pages9
JournalRSC Advances
Volume4
Issue number80
DOIs
StatePublished - 2014

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Immunosorbents
Bacteriophages
Pesticides
Peptides
Assays
Enzymes
Fenitrothion
Methyl Parathion
Peptide Library
Pesticide Residues
Catalyst selectivity
Gas chromatography
Display devices
Recovery

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Hua, X., Liu, X., Shi, H., Wang, Y., Kim, H. J., Gee, S. J., ... Hammock, B. D. (2014). Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. RSC Advances, 4(80), 42445-42453. https://doi.org/10.1039/c4ra07059c

Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. / Hua, Xiude; Liu, Xiaofeng; Shi, Haiyan; Wang, Yanru; Kim, Hee Joo; Gee, Shirley J.; Wang, Minghua; Liu, Fengquan; Hammock, Bruce D.

In: RSC Advances, Vol. 4, No. 80, 2014, p. 42445-42453.

Research output: Contribution to journalArticle

Hua, X, Liu, X, Shi, H, Wang, Y, Kim, HJ, Gee, SJ, Wang, M, Liu, F & Hammock, BD 2014, 'Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide', RSC Advances, vol. 4, no. 80, pp. 42445-42453. https://doi.org/10.1039/c4ra07059c
Hua, Xiude ; Liu, Xiaofeng ; Shi, Haiyan ; Wang, Yanru ; Kim, Hee Joo ; Gee, Shirley J. ; Wang, Minghua ; Liu, Fengquan ; Hammock, Bruce D. / Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. In: RSC Advances. 2014 ; Vol. 4, No. 80. pp. 42445-42453.
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