Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide

Xiude Hua, Xiaofeng Liu, Haiyan Shi, Yanru Wang, Hee Joo Kim, Shirley J. Gee, Minghua Wang, Fengquan Liu, Bruce D. Hammock

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to detect organophosphorus pesticides using a phage-borne peptide that was isolated from a cyclic 8-residue peptide phage library. The IC50 values of the phage ELISA ranged from 1.4 to 92.1 μg L-1 for eight organophosphorus pesticides (parathion-methyl, parathion, fenitrothion, cyanophos, EPN, paraoxon-methyl, paraoxon, fenitrooxon). The sensitivity was improved 120- and 2-fold compared to conventional homologous and heterologous ELISA, respectively. The selectivity of the phage ELISA was evaluated by measuring its cross-reactivity with 23 organophosphorus pesticides, among which eight were the main cross-reactants. The spike recoveries were between 66.1% and 101.6% for the detection of single pesticide residues of parathion-methyl, parathion and fenitrothion in Chinese cabbage, apple and greengroceries, and all of the coefficients of variation were less than or equal to 15.9%. Moreover, the phage ELISA results were validated by gas chromatography. The results indicate that isolating phage-borne peptides from phage display libraries is an alternative method for the development of a heterologous immunoassay and that the developed assay has a lower limit of detection than the chemically synthesized competitor assay.

Original languageEnglish (US)
Pages (from-to)42445-42453
Number of pages9
JournalRSC Advances
Volume4
Issue number80
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

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    Hua, X., Liu, X., Shi, H., Wang, Y., Kim, H. J., Gee, S. J., Wang, M., Liu, F., & Hammock, B. D. (2014). Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide. RSC Advances, 4(80), 42445-42453. https://doi.org/10.1039/c4ra07059c