Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor

N. Billinton, M. G. Barker, C. E. Michel, A. W. Knight, W. D. Heyer, N. J. Goddard, P. R. Fielden, R. M. Walmsley

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

A reporter system, constructed for a laboratory screen for new genes involved in DNA repair in the brewer's yeast Saccharomyces cerevisiae, has been developed for use in a genotoxicity biosensor. The strain produces green fluorescent protein (yEGFP) when DNA damage has occurred. yEGFP is codon optimised for yeasts. The reporter does not respond to chemicals which delay mitosis, and responds appropriately to the genetic regulation of DNA repair. Data is presented which demonstrate strain improvements appropriate to biosensor technology: improved signal to noise ratio, ease of data collection and uncomplicated material handling.

Original languageEnglish (US)
Pages (from-to)831-838
Number of pages8
JournalBiosensors and Bioelectronics
Volume13
Issue number7-8
DOIs
StatePublished - Oct 1998
Externally publishedYes

Keywords

  • Biosensor
  • DNA repair
  • Fluorescence
  • Genotoxicity
  • GFP
  • RAD54
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

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  • Cite this

    Billinton, N., Barker, M. G., Michel, C. E., Knight, A. W., Heyer, W. D., Goddard, N. J., Fielden, P. R., & Walmsley, R. M. (1998). Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor. Biosensors and Bioelectronics, 13(7-8), 831-838. https://doi.org/10.1016/S0956-5663(98)00049-9