Development of a confinable gene drive system in the human disease vector aedes aegypti

Ming Li, Ting Yang, Nikolay P. Kandul, Michelle Bui, Stephanie Gamez, Robyn Raban, Jared Bennett, Héctor M. Sánchez C, Gregory C. Lanzaro, Hanno Schmidt, Yoosook Lee, John M. Marshall, Omar S. Akbari

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect millions of people every year. With an escalating burden of infections and the relative failure of traditional control methods, the development of innovative control measures has become of paramount importance. The use of gene drives has sparked significant enthusiasm for genetic control of mosquitoes; however, no such system has been developed in Ae. aegypti. To fill this void, here we develop several CRISPR-based split gene drives for use in this vector. With cleavage rates up to 100% and transmission rates as high as 94%, mathematical models predict that these systems could spread anti-pathogen effector genes into wild populations in a safe, confinable and reversible manner appropriate for field trials and effective for controlling disease. These findings could expedite the development of effector-linked gene drives that could safely control wild populations of Ae. aegypti to combat local pathogen transmission.

Original languageEnglish (US)
Article numbere51701
JournaleLife
Volume9
DOIs
StatePublished - Jan 2020

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Li, M., Yang, T., Kandul, N. P., Bui, M., Gamez, S., Raban, R., Bennett, J., Sánchez C, H. M., Lanzaro, G. C., Schmidt, H., Lee, Y., Marshall, J. M., & Akbari, O. S. (2020). Development of a confinable gene drive system in the human disease vector aedes aegypti. eLife, 9, [e51701]. https://doi.org/10.7554/eLife.51701