Abstract
A general enzyme-linked immunosorbent assay was developed for the type I pyrethroid insecticides, such as permethrin, phenothrin, resmethrin and bioresmethrin. Polyclonal antibodies were generated by immunizing with a permethrin derivative, 2,2-dimethyl-3-(5′-carboxy-pent-1′-en-yl)cyclopropanecarboxylic acid-(3-phenoxybenzyl)ester conjugated with thyroglobulin, bovine serum albumin or ovalbumin. Antisera were screened against eight different coating antigens. The antibody-antigen combination with the lowest background, and highest sensitivity for permethrin was further optimized and tested for solvent and detergent tolerance. The I50's of the optimized immunoassay were 30 μg/l for permethrin and 20 μg/l for phenothrin, respectively. No cross-reactivities were measured to the type II pyrethroids, such as esfenvalerate, cyfluthrin, cypermethrin, deltamethrin, fenvalerate and fluvalinate. This assay can be used in monitoring studies to distinguish between types I and II pyrethroids.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 119-129 |
| Number of pages | 11 |
| Journal | Analytica Chimica Acta |
| Volume | 444 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 12 2001 |
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Keywords
- Class-specific immunoassay
- ELISA
- Permethrin
- Pyrethroid
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Development of a class-specific immunoassay for the type I pyrethroid insecticides. / Watanabe, Takaho; Shan, Guomin; Stoutamire, Donald W.; Gee, Shirley J.; Hammock, Bruce D.
In: Analytica Chimica Acta, Vol. 444, No. 1, 12.10.2001, p. 119-129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a class-specific immunoassay for the type I pyrethroid insecticides
AU - Watanabe, Takaho
AU - Shan, Guomin
AU - Stoutamire, Donald W.
AU - Gee, Shirley J.
AU - Hammock, Bruce D.
PY - 2001/10/12
Y1 - 2001/10/12
N2 - A general enzyme-linked immunosorbent assay was developed for the type I pyrethroid insecticides, such as permethrin, phenothrin, resmethrin and bioresmethrin. Polyclonal antibodies were generated by immunizing with a permethrin derivative, 2,2-dimethyl-3-(5′-carboxy-pent-1′-en-yl)cyclopropanecarboxylic acid-(3-phenoxybenzyl)ester conjugated with thyroglobulin, bovine serum albumin or ovalbumin. Antisera were screened against eight different coating antigens. The antibody-antigen combination with the lowest background, and highest sensitivity for permethrin was further optimized and tested for solvent and detergent tolerance. The I50's of the optimized immunoassay were 30 μg/l for permethrin and 20 μg/l for phenothrin, respectively. No cross-reactivities were measured to the type II pyrethroids, such as esfenvalerate, cyfluthrin, cypermethrin, deltamethrin, fenvalerate and fluvalinate. This assay can be used in monitoring studies to distinguish between types I and II pyrethroids.
AB - A general enzyme-linked immunosorbent assay was developed for the type I pyrethroid insecticides, such as permethrin, phenothrin, resmethrin and bioresmethrin. Polyclonal antibodies were generated by immunizing with a permethrin derivative, 2,2-dimethyl-3-(5′-carboxy-pent-1′-en-yl)cyclopropanecarboxylic acid-(3-phenoxybenzyl)ester conjugated with thyroglobulin, bovine serum albumin or ovalbumin. Antisera were screened against eight different coating antigens. The antibody-antigen combination with the lowest background, and highest sensitivity for permethrin was further optimized and tested for solvent and detergent tolerance. The I50's of the optimized immunoassay were 30 μg/l for permethrin and 20 μg/l for phenothrin, respectively. No cross-reactivities were measured to the type II pyrethroids, such as esfenvalerate, cyfluthrin, cypermethrin, deltamethrin, fenvalerate and fluvalinate. This assay can be used in monitoring studies to distinguish between types I and II pyrethroids.
KW - Class-specific immunoassay
KW - ELISA
KW - Permethrin
KW - Pyrethroid
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UR - http://www.scopus.com/inward/citedby.url?scp=0035851018&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(01)01163-1
DO - 10.1016/S0003-2670(01)01163-1
M3 - Article
AN - SCOPUS:0035851018
VL - 444
SP - 119
EP - 129
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -