Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro

David M. Roseman; Xiying Wu; Larry A. Milco; Mary Bober; R. Bryan Miller; Mark J. Kurth

Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro

David M. Roseman, Xiying Wu, Larry A. Milco, Mary Bober, R. Bryan Miller, Mark J. Kurth

Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I₅₀ value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.

Original language	English (US)
Pages (from-to)	1008-1014
Number of pages	7
Journal	Journal of Agricultural and Food Chemistry
Volume	40
Issue number	6
State	Published - 1992

ASJC Scopus subject areas

Agricultural and Biological Sciences (miscellaneous)
Food Science
Chemistry (miscellaneous)

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Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro. / Roseman, David M.; Wu, Xiying; Milco, Larry A.; Bober, Mary; Miller, R. Bryan; Kurth, Mark J.

In: Journal of Agricultural and Food Chemistry, Vol. 40, No. 6, 1992, p. 1008-1014.

Research output: Contribution to journal › Article › peer-review

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abstract = "A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.",

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AU - Roseman, David M.

AU - Wu, Xiying

AU - Milco, Larry A.

AU - Bober, Mary

AU - Miller, R. Bryan

AU - Kurth, Mark J.

PY - 1992

Y1 - 1992

N2 - A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.

AB - A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.

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