Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro

David M. Roseman, Xiying Wu, Larry A. Milco, Mary Bober, R. Bryan Miller, Mark J. Kurth

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.

Original languageEnglish (US)
Pages (from-to)1008-1014
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume40
Issue number6
StatePublished - 1992

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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