Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro

David M. Roseman, Xiying Wu, Larry A. Milco, Mary Bober, R. Bryan Miller, Mark J. Kurth

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.

Original languageEnglish (US)
Pages (from-to)1008-1014
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume40
Issue number6
StatePublished - 1992

Fingerprint

retronecine
Pyrrolizidine Alkaloids
pyrrolizidine alkaloids
Immunosorbents
Assays
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
retrorsine
Crotalaria retusa
Enzymes
Crotalaria
monocrotaline
Swainsonine
Senecio
swainsonine
Senecio vulgaris
Monocrotaline
haptens
Haptens
Plant Extracts

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro. / Roseman, David M.; Wu, Xiying; Milco, Larry A.; Bober, Mary; Miller, R. Bryan; Kurth, Mark J.

In: Journal of Agricultural and Food Chemistry, Vol. 40, No. 6, 1992, p. 1008-1014.

Research output: Contribution to journalArticle

Roseman, David M. ; Wu, Xiying ; Milco, Larry A. ; Bober, Mary ; Miller, R. Bryan ; Kurth, Mark J. / Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro. In: Journal of Agricultural and Food Chemistry. 1992 ; Vol. 40, No. 6. pp. 1008-1014.
@article{0c0983bfc4e149cd9321ef48efc64b53,
title = "Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro",
abstract = "A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.",
author = "Roseman, {David M.} and Xiying Wu and Milco, {Larry A.} and Mary Bober and Miller, {R. Bryan} and Kurth, {Mark J.}",
year = "1992",
language = "English (US)",
volume = "40",
pages = "1008--1014",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Development of a class-specific competitive enzyme-linked immunosorbent assay for the detection of pyrrolizidine alkaloids in vitro

AU - Roseman, David M.

AU - Wu, Xiying

AU - Milco, Larry A.

AU - Bober, Mary

AU - Miller, R. Bryan

AU - Kurth, Mark J.

PY - 1992

Y1 - 1992

N2 - A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.

AB - A two-step enzyme-linked immunosorbent assay (ELISA) was developed for the determination of the presence of retronecine in the parts per billion range. By modification of retronecine two haptens were synthesized. An I50 value of 11 ± 3 ppb (Figure 2a) was obtained, with detection limits of 1.0-100 ppb. Indirect detection of monocrotaline (325 g/mol), retrorsine (351 g/mol), and senecionine (352 g/mol) was achieved by hydrolytic digestion of these naturally occurring pyrrolizidine alkaloids (PAs), demonstrating the utility of the assay as a class-specific detection method. PAs were detected in Senecio vulgaris and Crotalaria retusa plant sample extracts. No cross-reactivity was seen with either swainsonine or lupinine, both of which are structurally similar to retronecine in that they are also fused bicyclic alkaloids.

UR - http://www.scopus.com/inward/record.url?scp=0001018771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001018771&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0001018771

VL - 40

SP - 1008

EP - 1014

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 6

ER -