Development of a class selective immunoassay for the type II pyrethroid insecticides

Sally K. Mak, Guomin Shan, Hu Jang Lee, Takaho Watanabe, Donald W. Stoutamire, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

A general and broad class selective enzyme-linked immunosorbent assay was developed for the type II pyrethroid insecticides, such as cypermethrin, deltamethrin, cyhalothrin, cyfluthrin, fenvalerate, esfenvalerate and fluvalinate. Polyclonal antibodies were generated by immunizing with a type II pyrethroid immunogen ((RS)-α-cyano-3-phenoxybenzyl (RS)-cis,trans-2,2- dimethyl-3-carboxyl-cyclopropanecarboxylate) conjugated with thyroglobulin. Antisera were screened against nine different coating antigens. The antibody-antigen combination with the most selectivity for type II pyrethroids such as cypermethrin was further optimized and tested for tolerance to co-solvent, pH and ionic strength changes. The IC50s of the optimized immunoassay were 78 μg l-1 for cypermethrin, 205 μg l -1 for cyfluthrin, 120 μg l-1 for cyhalothrin, 13 μg l-1 for deltamethrin, 6 μg l-1 for esfenvalerate, 8 μg l-1 for fenvalerate and 123 μg l-1 for fluvalinate. No cross-reactivity was measured for the type I pyrethroids such as permethrin, bifenthrin, phenothrin, resmethrin and bioresmethrin. This assay can be used in monitoring studies to distinguish between type I and II pyrethroids.

Original languageEnglish (US)
Pages (from-to)109-120
Number of pages12
JournalAnalytica Chimica Acta
Volume534
Issue number1
DOIs
StatePublished - Apr 4 2005

Fingerprint

fenvalerate
Pyrethrins
pyrethroid
immunoassay
Insecticides
Immunoassay
insecticide
cypermethrin
deltamethrin
phenothrin
antigen
antibody
Assays
assay
bifenthrin
Permethrin
Antigens
permethrin
Immunosorbents
Antibodies

Keywords

  • ELISA
  • Immunoassay
  • Insecticide
  • Pesticide class
  • Pyrethroid
  • Type II

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Mak, S. K., Shan, G., Lee, H. J., Watanabe, T., Stoutamire, D. W., Gee, S. J., & Hammock, B. D. (2005). Development of a class selective immunoassay for the type II pyrethroid insecticides. Analytica Chimica Acta, 534(1), 109-120. https://doi.org/10.1016/j.aca.2004.11.021

Development of a class selective immunoassay for the type II pyrethroid insecticides. / Mak, Sally K.; Shan, Guomin; Lee, Hu Jang; Watanabe, Takaho; Stoutamire, Donald W.; Gee, Shirley J.; Hammock, Bruce D.

In: Analytica Chimica Acta, Vol. 534, No. 1, 04.04.2005, p. 109-120.

Research output: Contribution to journalArticle

Mak, SK, Shan, G, Lee, HJ, Watanabe, T, Stoutamire, DW, Gee, SJ & Hammock, BD 2005, 'Development of a class selective immunoassay for the type II pyrethroid insecticides', Analytica Chimica Acta, vol. 534, no. 1, pp. 109-120. https://doi.org/10.1016/j.aca.2004.11.021
Mak, Sally K. ; Shan, Guomin ; Lee, Hu Jang ; Watanabe, Takaho ; Stoutamire, Donald W. ; Gee, Shirley J. ; Hammock, Bruce D. / Development of a class selective immunoassay for the type II pyrethroid insecticides. In: Analytica Chimica Acta. 2005 ; Vol. 534, No. 1. pp. 109-120.
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