Development of a class selective immunoassay for the type II pyrethroid insecticides

Sally K. Mak, Guomin Shan, Hu Jang Lee, Takaho Watanabe, Donald W. Stoutamire, Shirley J. Gee, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


A general and broad class selective enzyme-linked immunosorbent assay was developed for the type II pyrethroid insecticides, such as cypermethrin, deltamethrin, cyhalothrin, cyfluthrin, fenvalerate, esfenvalerate and fluvalinate. Polyclonal antibodies were generated by immunizing with a type II pyrethroid immunogen ((RS)-α-cyano-3-phenoxybenzyl (RS)-cis,trans-2,2- dimethyl-3-carboxyl-cyclopropanecarboxylate) conjugated with thyroglobulin. Antisera were screened against nine different coating antigens. The antibody-antigen combination with the most selectivity for type II pyrethroids such as cypermethrin was further optimized and tested for tolerance to co-solvent, pH and ionic strength changes. The IC50s of the optimized immunoassay were 78 μg l-1 for cypermethrin, 205 μg l -1 for cyfluthrin, 120 μg l-1 for cyhalothrin, 13 μg l-1 for deltamethrin, 6 μg l-1 for esfenvalerate, 8 μg l-1 for fenvalerate and 123 μg l-1 for fluvalinate. No cross-reactivity was measured for the type I pyrethroids such as permethrin, bifenthrin, phenothrin, resmethrin and bioresmethrin. This assay can be used in monitoring studies to distinguish between type I and II pyrethroids.

Original languageEnglish (US)
Pages (from-to)109-120
Number of pages12
JournalAnalytica Chimica Acta
Issue number1
StatePublished - Apr 4 2005


  • Immunoassay
  • Insecticide
  • Pesticide class
  • Pyrethroid
  • Type II

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry


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