Development of 21 microsatellite loci for puma (Puma concolor) ecology and forensics

Jennifer D. Kurushima, Julia A. Collins, Jay A. Well, Holly B Ernest

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We developed 21 microsatellite (13 dinucleotide and 8 tetranucleotide) primers specifically for pumas (Puma concolor). The primers were tested across 243 individuals from California and Nevada, and displayed an average of 5.5 alleles per locus. Previously, domestic cat (Felis catus) primers have been adapted for puma genetic studies. Puma-specific loci may reduce concerns associated with ascertainment bias, improve genetic structure resolution and provide additional tetranucleotide loci valuable for forensic applications. These puma-specific microsatellites will aid studies involving parentage, kinship and population structure, along with forensic case applications including poaching and puma-associated injuries to humans and domestic animals.

Original languageEnglish (US)
Pages (from-to)1260-1262
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number4
DOIs
StatePublished - Dec 2006

Fingerprint

Puma
Puma concolor
poaching
parentage
kinship
Ecology
Microsatellite Repeats
genetic structure
population structure
allele
microsatellite repeats
ecology
loci
animal
Animals
cats
Cats
domestic animals
Genetic Structures
Domestic Animals

Keywords

  • Forensics
  • Microsatellites
  • Mountain lion
  • Puma
  • Puma concolor

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Development of 21 microsatellite loci for puma (Puma concolor) ecology and forensics. / Kurushima, Jennifer D.; Collins, Julia A.; Well, Jay A.; Ernest, Holly B.

In: Molecular Ecology Notes, Vol. 6, No. 4, 12.2006, p. 1260-1262.

Research output: Contribution to journalArticle

Kurushima, Jennifer D. ; Collins, Julia A. ; Well, Jay A. ; Ernest, Holly B. / Development of 21 microsatellite loci for puma (Puma concolor) ecology and forensics. In: Molecular Ecology Notes. 2006 ; Vol. 6, No. 4. pp. 1260-1262.
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