Development of 21 microsatellite loci for puma (Puma concolor) ecology and forensics

Jennifer D. Kurushima, Julia A. Collins, Jay A. Well, Holly B Ernest

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We developed 21 microsatellite (13 dinucleotide and 8 tetranucleotide) primers specifically for pumas (Puma concolor). The primers were tested across 243 individuals from California and Nevada, and displayed an average of 5.5 alleles per locus. Previously, domestic cat (Felis catus) primers have been adapted for puma genetic studies. Puma-specific loci may reduce concerns associated with ascertainment bias, improve genetic structure resolution and provide additional tetranucleotide loci valuable for forensic applications. These puma-specific microsatellites will aid studies involving parentage, kinship and population structure, along with forensic case applications including poaching and puma-associated injuries to humans and domestic animals.

Original languageEnglish (US)
Pages (from-to)1260-1262
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number4
DOIs
StatePublished - Dec 2006

Keywords

  • Forensics
  • Microsatellites
  • Mountain lion
  • Puma
  • Puma concolor

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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