Development and validation of a quantitative PCR for rapid and specific detection of California sea lion adenovirus 1 and prevalence in wild and managed populations

Galaxia Cortés-Hinojosa, Frances M D Gulland, Tracey Goldstein, Stephanie Venn-Watson, Rebecca Rivera, Linda L. Archer, Thomas B. Waltzek, Gregory C. Gray, James F X Wellehan

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

California sea lion adenovirus 1 (CSLAdV-1) has been associated with hepatitis and enteritis in several wild and captive populations of diverse pinniped species. Currently available tests have been limited to pan-adenoviral polymerase chain reaction (PCR) followed by sequencing. We present the development of a quantitative probe-hybridization PCR (qPCR) assay for rapid, sensitive, and specific detection of this virus in California sea lions (Zalophus californianus) and other pinnipeds. This assay did not amplify other mammalian adenoviruses and is able to detect consistently down to 10 viral copies per well. Compared with the gold standard conventional pan-adenovirus PCR/sequencing assay, diagnostic sensitivity and specificity of 100% and 88.2% were found, respectively. The lower diagnostic specificity of this qPCR assay may be the result of the lower limit of detection of this assay compared with the gold standard rather than the result of detection of true false-positives.

Original languageEnglish (US)
Pages (from-to)193-197
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume29
Issue number2
DOIs
StatePublished - Mar 1 2017

Keywords

  • Adenovirus
  • California sea lions
  • pinnipeds
  • quantitative PCR

ASJC Scopus subject areas

  • veterinary(all)

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