Abstract
More sensitive tests are required for the diagnosis of Tritrichomonas foetus infection in cattle and an antigen-detecting enzyme immunoassay (EIA) has been applied to this purpose. An affinity purified immunoglobulin fraction obtained from rabbits immunised with cultured T. foetus served as both capture antibody and as biotinylated indicator antibody. While highly sensitive in the detection of antigen derived from cultured organisms, the assay showed poor sensitivity in the detection of antigen in the cervico-vaginal mucus of artificially infected heifers, with only 75% of culture-positive samples being considered positive for antigen. In a direct comparison, 23 122 samples from a naturally infected dairy herd gave positive cultures, while only 10 122 samples were considered antigen positive by EIA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 115-123 |
| Number of pages | 9 |
| Journal | Veterinary Parasitology |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1989 |
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ASJC Scopus subject areas
- Parasitology
- veterinary(all)
Cite this
Development and preliminary assessment of a polyclonal antibody-based enzyme immunoassay for the detection of Tritrichomonas foetus antigen in breeding cattle. / Yule, A.; Skirrow, S. Z.; Staats, J.; Bondurant, Robert.
In: Veterinary Parasitology, Vol. 31, No. 2, 01.01.1989, p. 115-123.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development and preliminary assessment of a polyclonal antibody-based enzyme immunoassay for the detection of Tritrichomonas foetus antigen in breeding cattle
AU - Yule, A.
AU - Skirrow, S. Z.
AU - Staats, J.
AU - Bondurant, Robert
PY - 1989/1/1
Y1 - 1989/1/1
N2 - More sensitive tests are required for the diagnosis of Tritrichomonas foetus infection in cattle and an antigen-detecting enzyme immunoassay (EIA) has been applied to this purpose. An affinity purified immunoglobulin fraction obtained from rabbits immunised with cultured T. foetus served as both capture antibody and as biotinylated indicator antibody. While highly sensitive in the detection of antigen derived from cultured organisms, the assay showed poor sensitivity in the detection of antigen in the cervico-vaginal mucus of artificially infected heifers, with only 75% of culture-positive samples being considered positive for antigen. In a direct comparison, 23 122 samples from a naturally infected dairy herd gave positive cultures, while only 10 122 samples were considered antigen positive by EIA.
AB - More sensitive tests are required for the diagnosis of Tritrichomonas foetus infection in cattle and an antigen-detecting enzyme immunoassay (EIA) has been applied to this purpose. An affinity purified immunoglobulin fraction obtained from rabbits immunised with cultured T. foetus served as both capture antibody and as biotinylated indicator antibody. While highly sensitive in the detection of antigen derived from cultured organisms, the assay showed poor sensitivity in the detection of antigen in the cervico-vaginal mucus of artificially infected heifers, with only 75% of culture-positive samples being considered positive for antigen. In a direct comparison, 23 122 samples from a naturally infected dairy herd gave positive cultures, while only 10 122 samples were considered antigen positive by EIA.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024671835&partnerID=8YFLogxK
U2 - 10.1016/0304-4017(89)90026-5
DO - 10.1016/0304-4017(89)90026-5
M3 - Article
VL - 31
SP - 115
EP - 123
JO - Veterinary Parasitology
JF - Veterinary Parasitology
SN - 0304-4017
IS - 2
ER -