Development and preliminary assessment of a polyclonal antibody-based enzyme immunoassay for the detection of Tritrichomonas foetus antigen in breeding cattle

A. Yule, S. Z. Skirrow, J. Staats, Robert Bondurant

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

More sensitive tests are required for the diagnosis of Tritrichomonas foetus infection in cattle and an antigen-detecting enzyme immunoassay (EIA) has been applied to this purpose. An affinity purified immunoglobulin fraction obtained from rabbits immunised with cultured T. foetus served as both capture antibody and as biotinylated indicator antibody. While highly sensitive in the detection of antigen derived from cultured organisms, the assay showed poor sensitivity in the detection of antigen in the cervico-vaginal mucus of artificially infected heifers, with only 75% of culture-positive samples being considered positive for antigen. In a direct comparison, 23 122 samples from a naturally infected dairy herd gave positive cultures, while only 10 122 samples were considered antigen positive by EIA.

Original languageEnglish (US)
Pages (from-to)115-123
Number of pages9
JournalVeterinary Parasitology
Volume31
Issue number2
DOIs
StatePublished - Jan 1 1989

Fingerprint

Tritrichomonas foetus
cattle breeding
enzyme immunoassays
polyclonal antibodies
Immunoenzyme Techniques
Breeding
antigen detection
antigens
Antigens
Antibodies
antibodies
mucus
dairy herds
sampling
immunoglobulins
heifers
rabbits
Mucus
cattle
organisms

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Development and preliminary assessment of a polyclonal antibody-based enzyme immunoassay for the detection of Tritrichomonas foetus antigen in breeding cattle. / Yule, A.; Skirrow, S. Z.; Staats, J.; Bondurant, Robert.

In: Veterinary Parasitology, Vol. 31, No. 2, 01.01.1989, p. 115-123.

Research output: Contribution to journalArticle

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