Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus)

T. P. O'Connor, S. Tanguay, R. Steinman, R. Smith, M. C. Barr, J. K. Yamamoto, Niels C Pedersen, P. R. Andersen, Q. J. Tonelli

Research output: Contribution to journalArticle

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Abstract

The feline T-lymphotropic lentivirus (feline immunodeficiency virus) is a recently described feline-specific retrovirus that can produce chronic immunodeficiency-like disorders in cats. A microdilution plate format enzyme-linked immunosorbent assay has been developed to detect the presence of antibody to the virus in feline serum of plasma. Temporal studies performed with experimentally infected animals show that seroconversion can be demonstrated 3 to 4 weeks after exposure to the virus. Results of a serosurvey (n = 1,556 samples) indicate that infection is fairly common in both clinic (5.2%) and sick cat (15.2%) populations. Western blot (immunoblot) and sodium dodecyl sulfate radioimmunoprecipitation assays were developed to confirm microdilution plate test results and to identify peptides specific for the feline immunodeficiency virus. All microdilution plate test positive results and selected negative results were confirmed by one or both of these procedures. These data demonstrate that this microassay plate enzyme-linked immunosorbent assay is a very sensitive and specific test for detection of antibody to the feline immunodeficiency virus.

Original languageEnglish (US)
Pages (from-to)474-479
Number of pages6
JournalJournal of Clinical Microbiology
Volume27
Issue number3
StatePublished - 1989
Externally publishedYes

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Feline Immunodeficiency Virus
Immunoassay
Antibodies
Felidae
Cats
Radioimmunoprecipitation Assay
Western Blotting
Enzyme-Linked Immunosorbent Assay
Viruses
Time and Motion Studies
Retroviridae
Sodium Dodecyl Sulfate
Peptides
Infection
Serum
Population

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

O'Connor, T. P., Tanguay, S., Steinman, R., Smith, R., Barr, M. C., Yamamoto, J. K., ... Tonelli, Q. J. (1989). Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus). Journal of Clinical Microbiology, 27(3), 474-479.

Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus). / O'Connor, T. P.; Tanguay, S.; Steinman, R.; Smith, R.; Barr, M. C.; Yamamoto, J. K.; Pedersen, Niels C; Andersen, P. R.; Tonelli, Q. J.

In: Journal of Clinical Microbiology, Vol. 27, No. 3, 1989, p. 474-479.

Research output: Contribution to journalArticle

O'Connor, TP, Tanguay, S, Steinman, R, Smith, R, Barr, MC, Yamamoto, JK, Pedersen, NC, Andersen, PR & Tonelli, QJ 1989, 'Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus)', Journal of Clinical Microbiology, vol. 27, no. 3, pp. 474-479.
O'Connor, T. P. ; Tanguay, S. ; Steinman, R. ; Smith, R. ; Barr, M. C. ; Yamamoto, J. K. ; Pedersen, Niels C ; Andersen, P. R. ; Tonelli, Q. J. / Development and evaluation of immunoassay for detection of antibodies to the feline T-lymphotropic lentivirus (feline immunodeficiency virus). In: Journal of Clinical Microbiology. 1989 ; Vol. 27, No. 3. pp. 474-479.
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AU - Barr, M. C.

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AU - Pedersen, Niels C

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