Development and characterization of recombinant virus generated from a New World Zika virus infectious clone

James Weger-Lucarelli, Nisha K. Duggal, Kristen Bullard-Feibelman, Milena Veselinovic, Hannah Romo, Chilinh Nguyen, Claudia Rückert, Aaron Brault, Richard A. Bowen, Mark Stenglein, Brian J. Geiss, Gregory D. Ebel

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. There are currently no molecular clones of a New World ZIKV available that lack significant attenuation, hindering progress toward understanding determinants of transmission and pathogenesis. Here we report the development and characterization of a novel ZIKV reverse genetics system based on a 2015 isolate from Puerto Rico (PRVABC59). We generated a two-plasmid infectious clone system from which infectious virus was rescued that replicates in human and mosquito cells with growth kinetics representative of wildtype ZIKV. Infectious clone-derived virus initiated infection and transmission rates in Aedes aegypti mosquitoes comparable to those of the primary isolate and displayed similar pathogenesis in AG129 mice. This infectious clone system provides a valuable resource to the research community to explore ZIKV molecular biology, vaccine development, antiviral development, diagnostics, vector competence, and disease pathogenesis.

Original languageEnglish (US)
Article numbere01765-16
JournalJournal of Virology
Volume91
Issue number1
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

Zika virus
Clone Cells
clones
Viruses
viruses
pathogenesis
Culicidae
Flaviviridae
Disease Vectors
Reverse Genetics
Flavivirus
Puerto Rico
vector competence
Synthetic Vaccines
Infectious Disease Transmission
Aedes
virus transmission
vaccine development
Virus Diseases
Aedes aegypti

Keywords

  • Flavivirus
  • Infectious clones
  • Zika virus

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Weger-Lucarelli, J., Duggal, N. K., Bullard-Feibelman, K., Veselinovic, M., Romo, H., Nguyen, C., ... Ebel, G. D. (2017). Development and characterization of recombinant virus generated from a New World Zika virus infectious clone. Journal of Virology, 91(1), [e01765-16]. https://doi.org/10.1128/JVI.01765-16

Development and characterization of recombinant virus generated from a New World Zika virus infectious clone. / Weger-Lucarelli, James; Duggal, Nisha K.; Bullard-Feibelman, Kristen; Veselinovic, Milena; Romo, Hannah; Nguyen, Chilinh; Rückert, Claudia; Brault, Aaron; Bowen, Richard A.; Stenglein, Mark; Geiss, Brian J.; Ebel, Gregory D.

In: Journal of Virology, Vol. 91, No. 1, e01765-16, 01.01.2017.

Research output: Contribution to journalArticle

Weger-Lucarelli, J, Duggal, NK, Bullard-Feibelman, K, Veselinovic, M, Romo, H, Nguyen, C, Rückert, C, Brault, A, Bowen, RA, Stenglein, M, Geiss, BJ & Ebel, GD 2017, 'Development and characterization of recombinant virus generated from a New World Zika virus infectious clone', Journal of Virology, vol. 91, no. 1, e01765-16. https://doi.org/10.1128/JVI.01765-16
Weger-Lucarelli J, Duggal NK, Bullard-Feibelman K, Veselinovic M, Romo H, Nguyen C et al. Development and characterization of recombinant virus generated from a New World Zika virus infectious clone. Journal of Virology. 2017 Jan 1;91(1). e01765-16. https://doi.org/10.1128/JVI.01765-16
Weger-Lucarelli, James ; Duggal, Nisha K. ; Bullard-Feibelman, Kristen ; Veselinovic, Milena ; Romo, Hannah ; Nguyen, Chilinh ; Rückert, Claudia ; Brault, Aaron ; Bowen, Richard A. ; Stenglein, Mark ; Geiss, Brian J. ; Ebel, Gregory D. / Development and characterization of recombinant virus generated from a New World Zika virus infectious clone. In: Journal of Virology. 2017 ; Vol. 91, No. 1.
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