Development and characterization of monoclonal antibodies directed against the nucleoprotein of heartland virus

Amanda E. Calvert, Aaron Brault

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Heartland virus (HRTV), a phlebovirus first isolated from two Missouri farmers in 2009, has been proposed to be transmitted to humans by the bite of infected Amblyomma americanum ticks. It is closely related to severe fever with thrombocytopenia syndrome virus (SFTSV) from China, another previously unrecognized phlebovirus that has subsequently been associated with hundreds of cases of severe disease in humans. To expand diagnostic capacity to detect HRTV infections, 20 hybridoma clones secreting anti-HRTV murine monoclonal antibodies (MAbs) were developed using splenocytes from HRTV-inoculated AG129 alpha/beta and gamma interferon receptor-deficient mice. Nine of these MAbs were characterized herein for inclusion in future HRTV diagnostic assay development. All of the MAbs developed were found to be non-neutralizing and reactive to linear epitopes on HRTV nucleocapsid protein. MAb 2AF11 was found to be cross-reactive with SFTSV.

Original languageEnglish (US)
Pages (from-to)1338-1340
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume93
Issue number6
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology
  • Medicine(all)

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