Development and characterization of an ELISA for trans-3-hydroxycotinine, a biomarker for mainstream and sidestream smoke exposure

W. A. Rees, S. Kwiatkowski, Scott D Stanley, D. E. Granstrom, J. M. Yang, C. G. Gairola, D. Drake, J. Glowczyk, W. E. Woods, T. Tobin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

trans-3-Hydroxycotinine is the major urinary metabolite of nicotine in man and can serve as an important biomarker of tobacco smoke exposure. A sensitive ELISA test for trans-3-hydroxycotinine was developed with an 1-50 for this nicotine biomarker of between 1.0-3.0 ng/ml. This ELISA test has about 10 fold less affinity for cotinine and 1000-fold less affinity for nicotine and other nicotine metabolites. No matrix effects were detectable in human saliva and relatively small matrix effects (I-50 for trans-3-hydroxycotinine, about 25 ng/ml) in urine was observed. The assay readily detected levels of apparent trans-3-hydroxycotinine in urine samples from smoke-exposed mice and rats. This ELISA is therefore a sensitive test for the determination of trans-3-hydroxycotinine in plasma, saliva, and urine samples from humans and animals, and can be used to monitor exposure to tobacco smoke or nicotine.

Original languageEnglish (US)
Title of host publicationASTM Special Technical Publication
PublisherAmerican Society for Testing and Materials
Pages149-162
Number of pages14
Volume1306
StatePublished - 1996
Externally publishedYes

Keywords

  • Cotinine
  • Enzyme-Linked Immunosorbant Assay
  • Metabolite
  • Nicotine
  • t-3-hydroxycotinine

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint Dive into the research topics of 'Development and characterization of an ELISA for trans-3-hydroxycotinine, a biomarker for mainstream and sidestream smoke exposure'. Together they form a unique fingerprint.

  • Cite this

    Rees, W. A., Kwiatkowski, S., Stanley, S. D., Granstrom, D. E., Yang, J. M., Gairola, C. G., Drake, D., Glowczyk, J., Woods, W. E., & Tobin, T. (1996). Development and characterization of an ELISA for trans-3-hydroxycotinine, a biomarker for mainstream and sidestream smoke exposure. In ASTM Special Technical Publication (Vol. 1306, pp. 149-162). American Society for Testing and Materials.